Anti-ATF2 (phospho S490 + S498)抗体(ab22186)

概述

  • 产品名称Anti-ATF2 (phospho S490 + S498)抗体
    参阅全部 ATF2 一抗
  • 描述
    兔多克隆抗体to ATF2 (phospho S490 + S498)
  • 特异性This antibody is specific for ~74 kDa ATF2 protein phosphorylated at Ser 490/498. The antibody also recognizes the phosphorylated ~54 kDa splice form of ATF2.
  • 经测试应用适用于: IHC-Fr, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic phosphopeptide corresponding to amino acid residues surrounding the phospho Ser490/498 of ATF2 (Human).

  • 阳性对照
    • Human melanoma cells.

性能

应用

Our Abpromise guarantee covers the use of ab22186 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/1000.
WB 1/1000. Predicted molecular weight: 52 kDa.

靶标

  • 功能Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
  • 组织特异性Abundant expression seen in the brain.
  • 序列相似性Belongs to the bZIP family. ATF subfamily.
    Contains 1 bZIP domain.
    Contains 1 C2H2-type zinc finger.
  • 翻译后修饰Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Activating transcription factor 2 antibody
    • Activating transcription factor 2 splice variant ATF2 var2 antibody
    • ATF 2 antibody
    • Atf-2 antibody
    • Atf2 antibody
    • ATF2 protein antibody
    • ATF2_HUMAN antibody
    • cAMP Response Element Binding Protein 2 antibody
    • cAMP response element binding protein CRE BP1 antibody
    • cAMP response element-binding protein CRE-BP1 antibody
    • cAMP responsive element binding protein 2, formerly antibody
    • cAMP-dependent transcription factor ATF-2 antibody
    • cAMP-responsive element-binding protein 2 antibody
    • CRE BP1 antibody
    • CRE-BP antibody
    • CREB 2 antibody
    • CREB-2 antibody
    • CREB2 antibody
    • CREBP1 antibody
    • Cyclic AMP dependent transcription factor ATF 2 antibody
    • Cyclic AMP-dependent transcription factor ATF-2 antibody
    • Cyclic AMP-responsive element-binding protein 2 antibody
    • D130078H02Rik antibody
    • D18875 antibody
    • HB 16 antibody
    • HB16 antibody
    • Histone acetyltransferase ATF2 antibody
    • MGC105211 antibody
    • MGC105222 antibody
    • MGC111558 antibody
    • MGC142504 antibody
    • mXBP antibody
    • MXBP protein antibody
    • Tg(Gzma-Klra1)7Wum antibody
    • TREB 7 antibody
    • TREB7 antibody
    see all

Anti-ATF2 (phospho S490 + S498) antibody (ab22186)参考文献

ab22186 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HaCAT (keratinocytes))
Loading amount 50 µg
Specification HaCAT (keratinocytes)
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
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提交于 May 05 2009

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