概述

  • 产品名称
  • 描述
    兔多克隆抗体to ATF2
  • 特异性
    ATF2 antibody detects endogenous levels of ATF2 protein around Threonine 71 or 53.
  • 经测试应用
    适用于: WB, IHC-P, ELISA, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    The antiserum was produced against synthesized non-phosphopeptide derived from human ATF2 around the phosphorylation site of Threonine 71 or 53.

性能

应用

Our Abpromise guarantee covers the use of ab47476 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 52 kDa.
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.
IP Use at an assay dependent concentration.

靶标

  • 功能
    Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
  • 组织特异性
    Abundant expression seen in the brain.
  • 序列相似性
    Belongs to the bZIP family. ATF subfamily.
    Contains 1 bZIP domain.
    Contains 1 C2H2-type zinc finger.
  • 翻译后修饰
    Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Activating transcription factor 2 antibody
    • Activating transcription factor 2 splice variant ATF2 var2 antibody
    • ATF 2 antibody
    • Atf-2 antibody
    • Atf2 antibody
    • ATF2 protein antibody
    • ATF2_HUMAN antibody
    • cAMP Response Element Binding Protein 2 antibody
    • cAMP response element binding protein CRE BP1 antibody
    • cAMP response element-binding protein CRE-BP1 antibody
    • cAMP responsive element binding protein 2, formerly antibody
    • cAMP-dependent transcription factor ATF-2 antibody
    • cAMP-responsive element-binding protein 2 antibody
    • CRE BP1 antibody
    • CRE-BP antibody
    • CREB 2 antibody
    • CREB-2 antibody
    • CREB2 antibody
    • CREBP1 antibody
    • Cyclic AMP dependent transcription factor ATF 2 antibody
    • Cyclic AMP-dependent transcription factor ATF-2 antibody
    • Cyclic AMP-responsive element-binding protein 2 antibody
    • D130078H02Rik antibody
    • D18875 antibody
    • HB 16 antibody
    • HB16 antibody
    • Histone acetyltransferase ATF2 antibody
    • MGC105211 antibody
    • MGC105222 antibody
    • MGC111558 antibody
    • MGC142504 antibody
    • mXBP antibody
    • MXBP protein antibody
    • Tg(Gzma-Klra1)7Wum antibody
    • TREB 7 antibody
    • TREB7 antibody
    see all

Anti-ATF2 antibody 图像

  • All lanes : Anti-ATF2 antibody (ab47476) at 1/500 dilution

    Lane 1 : extracts from HeLa cells.
    Lane 2 : extracts from HeLa cells preincubated with immunizing peptide


    Predicted band size : 52 kDa
    Observed band size : 52 kDa
  • Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left: Using ATF2 antibody; Right: The same antibody preincubated with synthesized peptide.

Anti-ATF2 antibody (ab47476)参考文献

This product has been referenced in:
  • Liu Z  et al. Adiponectin reduces ER stress-induced apoptosis through PPARa transcriptional regulation of ATF2 in mouse adipose. Cell Death Dis 7:e2487 (2016). WB ; Mouse . Read more (PubMed: 27882945) »
  • Marin TL  et al. Identification of AMP-activated protein kinase targets by a consensus sequence search of the proteome. BMC Syst Biol 9:13 (2015). WB . Read more (PubMed: 25890336) »

See all 4 Publications for this product

Product Wall

Thank you for your enquiry regarding ab31419 and ab47476; and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is having problems with these two antibodies.
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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (endothelial)
Loading amount
5000 cells
Specification
endothelial
Treatment
different shear stress exposure for 24 hours
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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提交于 Mar 01 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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