Anti-ATF2抗体(ab47476)
Key features and details
- Rabbit polyclonal to ATF2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-ATF2抗体
参阅全部 ATF2 一抗 -
描述
兔多克隆抗体to ATF2 -
宿主
Rabbit -
特异性
ATF2 antibody detects endogenous levels of ATF2 protein around Threonine 71 or 53. -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human ATF2 aa 38-87.
Database link: P15336 -
阳性对照
- WB: Extracts from HeLa cells. IHC-P: Human breast carcinoma.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading...
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克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Corresponding phospho antibody
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab47476于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 52 kDa.
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IHC-P |
Use at an assay dependent concentration.
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说明 |
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WB
1/500 - 1/1000. Predicted molecular weight: 52 kDa. |
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex. -
组织特异性
Abundant expression seen in the brain. -
序列相似性
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Contains 1 C2H2-type zinc finger. -
翻译后修饰
Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 1386 Human
- Omim: 123811 Human
- SwissProt: P15336 Human
- Unigene: 592510 Human
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别名
- Activating transcription factor 2 antibody
- Activating transcription factor 2 splice variant ATF2 var2 antibody
- ATF 2 antibody
see all
图片
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All lanes : Anti-ATF2 antibody (ab47476) at 1/500 dilution
Lane 1 : extracts from HeLa cells.
Lane 2 : extracts from HeLa cells preincubated with immunizing peptide
Predicted band size: 52 kDa
Observed band size: 52 kDa -
Immunohistochemical analysis of paraffin-embedded human breast carcinoma. Left: Using ATF2 antibody; Right: The same antibody preincubated with synthesized peptide.
数据表及文件
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SDS download
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Datasheet download
文献 (12)
ab47476 被引用在 12 文献中.
- Hu X et al. Ubiquitin fold modifier 1 activates NF-?B pathway by down-regulating LZAP expression in the macrophage of diabetic mouse model. Biosci Rep 40:N/A (2020). PubMed: 31829413
- Han F et al. Exosomal microRNA-26b-5p down-regulates ATF2 to enhance radiosensitivity of lung adenocarcinoma cells. J Cell Mol Med 24:7730-7742 (2020). PubMed: 32476275
- Gao T et al. KIF18B promotes tumor progression in osteosarcoma by activating ß-catenin. Cancer Biol Med 17:371-386 (2020). PubMed: 32587775
- Li P et al. Inhibition of long noncoding RNA HIF1A-AS2 confers protection against atherosclerosis via ATF2 downregulation. J Adv Res 26:123-135 (2020). PubMed: 33133688
- Zhou Y et al. Ligustilide attenuates nitric oxide-induced apoptosis in rat chondrocytes and cartilage degradation via inhibiting JNK and p38 MAPK pathways. J Cell Mol Med 23:3357-3368 (2019). PubMed: 30770640