Anti-ATF1 (phospho S63)抗体[EP1591(2)Y] (ab76085)


  • 产品名称Anti-ATF1 (phospho S63)抗体[EP1591(2)Y]
    参阅全部 ATF1 一抗
  • 描述
    兔单克隆抗体[EP1591(2)Y] to ATF1 (phospho S63)
  • 特异性ab76085 detects ATF1 when phosphorylated at serine 63
  • 经测试应用适用于: ELISA, WB, IHC-P, ICCmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A phospho specific peptide corresponding to residues surrounding serine 63 of human ATF1

  • 阳性对照
    • WB: HeLa cell lysate IHC-P: human breast carcinoma
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.




Our Abpromise guarantee covers the use of ab76085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration. PubMed: 22052915
WB 1/50000 - 1/100000. Predicted molecular weight: 29 kDa.
IHC-P 1/100 - 1/250.
ICC 1/100 - 1/250.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes.
    • 疾病相关Defects in ATF1 may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=Chromosomal aberrations involving ATF1 are found in patients with angiomatoid fibrous histiocytoma. Translocation t(12;16)(q13;p11.2) with FUS generates a chimeric ATF1/FUS protein. Translocation t(12;22)(q13;q12) with EWSR1 generates a chimeric ATF1/EWSR1 protein.
    • 序列相似性Belongs to the bZIP family. ATF subfamily.
      Contains 1 bZIP domain.
      Contains 1 KID (kinase-inducible) domain.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Activating transcription factor 1 antibody
      • ATF 1 antibody
      • atf1 antibody
      • ATF1 EWS fusion gene antibody
      • ATF1 FUS fusion gene antibody
      • ATF1_HUMAN antibody
      • cAMP dependent transcription factor 1 antibody
      • cAMP-dependent transcription factor ATF-1 antibody
      • Cyclic AMP dependent transcription factor ATF 1 antibody
      • Cyclic AMP dependent transcription factor ATF1 antibody
      • Cyclic AMP-dependent transcription factor ATF-1 antibody
      • EWS AFT1 antibody
      • FUS ATF 1 antibody
      • FUS/ATF 1 antibody
      • Protein TREB36 antibody
      • RNA binding protein activating transcription factor 1 fusion protein antibody
      • TREB 36 antibody
      • TREB36 antibody
      • TREB36 protein antibody
      see all

    Anti-ATF1 (phospho S63) antibody [EP1591(2)Y] 图像

    • All lanes : Anti-ATF1 (phospho S63) antibody [EP1591(2)Y] (ab76085) at 1/100000 dilution

      Lane 1 : HeLa cell lysate (untreated)
      Lane 2 : HeLa cell lysate, treated with Lambda Phosphatase

      Lysates/proteins at 10 µg per lane.

      goat anit rabbit HRP labelled IgG at 1/2000 dilution

      Predicted band size : 29 kDa
      Observed band size : 29 kDa
    • Immunohistochemical analysis of ATF1 in paraffin embedded human breast carcinoma tissue, using ab76085 at a 1/100 dilution.

    Anti-ATF1 (phospho S63) antibody [EP1591(2)Y] (ab76085)参考文献

    This product has been referenced in:
    • Lee CJ  et al. Fibroblast and Epidermal Growth Factors Utilize Different Signaling Pathways to Induce Anchorage-independent Cell Transformation in JB6 Cl41 Mouse Skin Epidermal Cells. J Cancer Prev 19:199-208 (2014). Read more (PubMed: 25337589) »
    • Shihan M  et al. Dehydroepiandrosterone sulfate mediates activation of transcription factors CREB and ATF-1 via a Ga11-coupled receptor in the spermatogenic cell line GC-2. Biochim Biophys Acta 1833:3064-3075 (2013). ICC/IF ; Human . Read more (PubMed: 23988737) »

    See all 3 Publications for this product

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    The concentration of ab76085 batch:GR68333 is 0.2150 mg/ml.

    This a tissue culture supernatant and not affinity purified antibody. As the datasheet indicates ab76085 is a rabbit monoclonal antibody which is...

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