Anti-ATF1抗体(ab66016)

概述

  • 产品名称Anti-ATF1抗体
    参阅全部 ATF1 一抗
  • 描述
    兔多克隆抗体to ATF1
  • 特异性ab66016 detects endogenous levels of total ATF1 protein.
  • 经测试应用适用于: ELISA, ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from an internal sequence of human ATF1.

  • 阳性对照
    • ICC/IF: HUVEC cells WB: Extracts from COLO205 cells, HeLa cells and HepG2 cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab66016 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab66016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.
WB 1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 29 kDa).

靶标

  • 功能This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes.
  • 疾病相关Defects in ATF1 may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=Chromosomal aberrations involving ATF1 are found in patients with angiomatoid fibrous histiocytoma. Translocation t(12;16)(q13;p11.2) with FUS generates a chimeric ATF1/FUS protein. Translocation t(12;22)(q13;q12) with EWSR1 generates a chimeric ATF1/EWSR1 protein.
  • 序列相似性Belongs to the bZIP family. ATF subfamily.
    Contains 1 bZIP domain.
    Contains 1 KID (kinase-inducible) domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Activating transcription factor 1 antibody
    • ATF 1 antibody
    • atf1 antibody
    • ATF1 EWS fusion gene antibody
    • ATF1 FUS fusion gene antibody
    • ATF1_HUMAN antibody
    • cAMP dependent transcription factor 1 antibody
    • cAMP-dependent transcription factor ATF-1 antibody
    • Cyclic AMP dependent transcription factor ATF 1 antibody
    • Cyclic AMP dependent transcription factor ATF1 antibody
    • Cyclic AMP-dependent transcription factor ATF-1 antibody
    • EWS AFT1 antibody
    • FUS ATF 1 antibody
    • FUS/ATF 1 antibody
    • Protein TREB36 antibody
    • RNA binding protein activating transcription factor 1 fusion protein antibody
    • TREB 36 antibody
    • TREB36 antibody
    • TREB36 protein antibody
    see all

Anti-ATF1 antibody 图像

  • All lanes : Anti-ATF1 antibody (ab66016) at 1/500 dilution

    Lane 1 : COLO205 cell extracts
    Lane 2 : HeLa cell extracts
    Lane 3 : HepG2 cell extracts
    Lane 4 : HepG2 cell extracts with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 29 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • ab66016 at 1/500 dilution staining ATF1 in HUVEC cells by Immunofluorescence, in the absence or presence of the immunising peptide.

Anti-ATF1 antibody (ab66016)参考文献

ab66016 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH 6.0
Sample Mouse Tissue sections (Embryonic 14-day-old brain, 16 micron)
Specification Embryonic 14-day-old brain, 16 micron
Permeabilization Yes - Triton X100, 0.5%
Fixative Paraformaldehyde
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Abcam user community

Verified customer

提交于 Feb 04 2015

Thank you for contacting Abcam.

Please find attached a copy of the alignment between the bovine and human sequences. Based on the level of homolgy between, I do not think that the antibody ab6616 would react with bovine tissue.

Plea...

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