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Our Abpromise guarantee covers the use of ab28830 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|ICC/IF||Use at an assay dependent concentration.|
ab28830 staining ATF-4 (phospho S245) in Human MRC5 cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 pH 7.4 for 5 minutes at room temperature and blocked with 5% BSA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/100 in PBS) for 1 hour. A CF488A-conjugated Donkey anti-rabbit IgG polyclonal was used as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"