Anti-Ataxin 7抗体(ab11434)

概述

  • 产品名称Anti-Ataxin 7抗体
    参阅全部 Ataxin 7 一抗
  • 描述
    兔多克隆抗体to Ataxin 7
  • 经测试应用适用于: ICC/IF, ICC, IP, IHC-Fr, WBmore details
  • 种属反应性
    与反应: Human, Drosophila melanogaster
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide corresponding to Human Ataxin 7 aa 1-17.
    Sequence:

    MSERAADDVRGEPRRAA


    (Peptide available as ab39761)

  • 阳性对照
    • Whole cell lysate from COS-1 cells transfected with Ataxin 7.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab11434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
ICC 1/500.
IP Use at an assay dependent concentration.
IHC-Fr 1/500 - 1/1000.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 96 kDa.Can be blocked with Human Ataxin 7 peptide (ab39761).

靶标

  • 相关性Ataxin 7 is a protein of unknown function. It may be the human orthologue of the yeast SAGA SGF73 subunit and a subunit of the human TFTC-like transcriptional complexes. Spinocerebellar ataxia 7 (one of a group of hereditary neurodegenrative diseases) is caused by an expanded trinucleotide repeat in the gene encoding ataxin 7. Ataxin 7 is typically located in the cytoplasm and on the nuclear membrane of normal brain neurons. In cells where there is a mutation of the SCA7 gene, ataxin 7 accumulates in intranuclear inclusions and can result in cell death.
  • 细胞定位Cytoplasmic (isoform b) and Nuclear (isoform a)
  • 数据库链接
  • 别名
    • ADCAII antibody
    • ATXN 7 antibody
    • OPCA III antibody
    • OPCA3 antibody
    • SCA 7 antibody
    • SCA7 antibody
    • Spinocerebellar Ataxia 7 antibody
    • Spinocerebellar ataxia type 7 protein antibody
    see all

Anti-Ataxin 7 antibody 图像

  • Western blot of Ataxin 7 in transfected COS-1 cell lysate using ab11434.
  • ICC/IF image of ab11434 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11434, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Ataxin 7 antibody (ab11434)参考文献

ab11434 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Brain)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 20 µg
Specification Brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Oct 21 2016

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