概述

  • 产品名称Anti-Atad5抗体
    参阅全部 Atad5 一抗
  • 描述
    兔多克隆抗体to Atad5
  • 经测试应用适用于: ICC/IF, WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Gorilla
  • 免疫原

    Synthetic peptide corresponding to a region between residues 775 and 825 of human Atad5 (NP_079133.3)

  • 阳性对照
    • HeLa and 293T whole cell lysates.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab72111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 22493068
WB 1/2000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 208 kDa).

One of our customer have mentioned using WB protocol published in J. Biol. Chem. 2010, 285:10362-10369 with lymphoblastoid cell.

IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能Involved in DNA damage response. Involved in a RAD9A-related damage checkpoint, a pathway that is important in determining whether DNA damage is compatible with cell survival or whether it requires cell elimination by apoptosis. Modulates the RAD9A interaction with BCL2 and thereby induces DNA damages-induced apoptosis.
  • 序列相似性Belongs to the AAA ATPase family.
  • 翻译后修饰ATR may stimulate the RAD9A dissociation.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATAD5 antibody
    • ATAD5_HUMAN antibody
    • ATPase family AAA domain containing 5 antibody
    • ATPase family AAA domain-containing protein 5 antibody
    • C17orf41 antibody
    • chromosome fragility associated gene 1 antibody
    • Chromosome fragility-associated gene 1 protein antibody
    • ELG1 antibody
    • Enhanced level of genomic instability 1 homolog antibody
    • FRAG1 antibody
    see all

Anti-Atad5 antibody 图像

  • All lanes : Anti-Atad5 antibody (ab72111) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg/ml
    Lane 2 : HeLa whole cell lysate at 15 µg/ml
    Lane 3 : HeLa whole cell lysate at 5 µg/ml
    Lane 4 : 293T whole cell lysate at 50 µg/ml

    Developed using the ECL technique

    Predicted band size : 208 kDa
    Observed band size : 160 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds
  • Detection of Atad5 by Western Blot of Immunprecipitate.
    ab72111 at 1µg/ml staining Atad5 in HeLa whole cell lysate immunoprecipitated using ab72111 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

Anti-Atad5 antibody (ab72111)参考文献

This product has been referenced in:
  • Shiomi Y  et al. Two different replication factor C proteins, Ctf18 and RFC1, separately control PCNA-CRL4Cdt2-mediated Cdt1 proteolysis during S phase and following UV irradiation. Mol Cell Biol 32:2279-88 (2012). ICC/IF ; Human . Read more (PubMed: 22493068) »
  • Rahman S  et al. The Brd4 extraterminal domain confers transcription activation independent of pTEFb by recruiting multiple proteins, including NSD3. Mol Cell Biol 31:2641-52 (2011). Read more (PubMed: 21555454) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100 in PBS
Username

Dr. Kirk McManus

Verified customer

提交于 Feb 11 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"