Anti-ATAD1抗体[N125/10] (ab94583)

概述

  • 产品名称
    Anti-ATAD1抗体[N125/10]
    参阅全部 ATAD1 一抗
  • 描述
    小鼠单克隆抗体[N125/10] to ATAD1
  • 经测试应用
    适用于: WB, IHC-P, IHC-Fr, ICC, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Fusion protein, corresponding to amino acids 1-361 (full length) of Mouse ATAD1

  • 阳性对照
    • Rat brain lysate
  • 常规说明

    The clone number has been updated from S125-10 to N125/10, both clone numbers name the same antibody clone.

     

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    单克隆
  • 克隆编号
    N125/10
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab94583 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
IHC-Fr
ICC
ICC/IF
  • 应用说明
    ICC: Use at a concentration of 0.1 - 1 µg/ml.
    ICC/IF: Use at a concentration of 1 - 10 µg/ml.
    IHC-P: Use at a concentration of 0.1 - 1 µg/ml.
    IHC-Fr: Use at a concentration of 0.1 - 1 µg/ml.
    WB: Use at a concentration of 1 - 10 µg/ml. Predicted molecular weight: 40 kDa.
    Note: Using the lysate without boiling may provide improved results.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      ATPase that plays a critical role in regulating the surface expression of AMPA receptors (AMPAR), thereby regulating synaptic plasticity and learning and memory. Required for NMDA-stimulated AMPAR internalization and inhibition of GRIA1 and GRIA2 recycling back to the plasma membrane; these activities are ATPase-dependent.
    • 序列相似性
      Belongs to the AAA ATPase family.
    • 细胞定位
      Peroxisome. Cell junction, synapse, postsynaptic cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 4921525H23Rik antibody
      • AAA+ ATPase Thorase antibody
      • Ab2-088 antibody
      • AFDC1 antibody
      • Atad1 antibody
      • ATAD1_HUMAN antibody
      • ATPase family AAA domain containing protein 1 antibody
      • ATPase family AAA domain-containing protein 1 antibody
      • ATPase family, AAA domain containing 1 antibody
      • ATPase Thorase antibody
      • AW107648 antibody
      • FLJ14600 antibody
      • FNP001 antibody
      • Thorase antibody
      see all

    图片



    • Predicted band size : 40 kDa
      Western blot detection of Atad1 in Rat brain membrane tissues using ab94583 at 1:1000 dilution.

    文献

    ab94583 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293T)
    Loading amount
    25 µg
    Specification
    293T
    Treatment
    siRNA knockdown
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Oct 08 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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