Anti-ASXL1抗体(ab55825)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to ASXL1
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Recombinant fragment
    预测可用于: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to C-terminal residues of human ASXL1.

性能

应用

Our Abpromise guarantee covers the use of ab55825 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 165 kDa. for 2 hours. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA Use at an assay dependent dilution.

靶标

  • 功能
    Probable Polycomb group (PcG) protein. Non-catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1).
  • 组织特异性
    Widely expressed at low level. Expressed in heart, brain, skeletal muscle, placenta, pancreas, spleen, prostate, small intestine, colon, peripheral blood, leukocytes, bone marrow and fetal liver. Highly expressed in testes.
  • 序列相似性
    Belongs to the Asx family.
    Contains 1 PHD-type zinc finger.
  • 结构域
    Contains one Leu-Xaa-Xaa-Leu-Leu (LXXLL) motif, which may be required for an association with nuclear receptors.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Additional sex combs like 1 (Drosophila) antibody
    • Additional sex combs like protein 1 antibody
    • Additional sex combs-like protein 1 antibody
    • ASXL1 antibody
    • ASXL1_HUMAN antibody
    • KIAA0978 antibody
    • Putative Polycomb group protein ASXL1 antibody
    see all

实验方案

文献

ab55825 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Leukemia Cell Lines)
Loading amount
60 µg
Specification
Leukemia Cell Lines
Gel Running Conditions
Non-reduced Denaturing (7.5%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dipmoy Nath

Verified customer

提交于 Sep 19 2012

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I am very pleased to hear you would like to accept our offer and test ab55825 in mouse and/or human. This code will give you: 1 free PRIMARY ANTIBODY befor...

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