概述

  • 产品名称
    Anti-Asporin抗体
    参阅全部 Asporin 一抗
  • 描述
    兔多克隆抗体to Asporin
  • 经测试应用
    适用于: WB, IHC-P, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    A region within synthetic peptide: ISTVELEDFK RYKELQRLGL GNNKITDIEN GSLANIPRVR EIHLENNKLK, corresponding to internal sequence amino acids 253-302 of Human Asporin

  • 阳性对照
    • HepG2 cell lysate or human muscle tissue.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab58741 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 43 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能
    Negatively regulates periodontal ligament (PDL) differentiation and mineralization to ensure that the PDL is not ossified and to maintain homeostasis of the tooth-supporting system. Inhibits BMP2-induced cytodifferentiation of PDL cells by preventing its binding to BMPR1B/BMP type-1B receptor, resulting in inhibition of BMP-dependent activation of SMAD proteins (By similarity). Critical regulator of TGF-beta in articular cartilage and plays an essential role in cartilage homeostasis and osteoarthritis (OA) pathogenesis. Negatively regulates chondrogenesis in the articular cartilage by blocking the TGF-beta/receptor interaction on the cell surface and inhibiting the canonical TGF-beta/Smad signal. Binds calcium and plays a role in osteoblast-driven collagen biomineralization activity.
  • 组织特异性
    Higher levels in osteoarthritic articular cartilage, aorta, uterus. Moderate expression in small intestine, heart, liver, bladder, ovary, stomach, and in the adrenal, thyroid, and mammary glands. Low expression in trachea, bone marrow, and lung. Co-localizes with TGFB1 in chondrocytes within osteoarthritic (OA) lesions of articular cartilage.
  • 疾病相关
    Genetic variations in ASPN are associated with susceptibility to osteoarthritis type 3 (OS3) [MIM:607850]; also known as osteoarthritis of knee/hip. Osteoarthritis is a degenerative disease of the joints characterized by degradation of the hyaline articular cartilage and remodeling of the subchondral bone with sclerosis. Clinical symptoms include pain and joint stiffness often leading to significant disability and joint replacement. Note=Susceptibility to osteoarthritis is conferred by a triplet repeat expansion polymorphism. ASPN allele having 14 aspartic acid repeats in the N-terminal region of the protein (D14), is overrepresented relative to the common allele having 13 aspartic acid repeats (D13). The frequency of the D14 allele increases with disease severity. The D14 allele is also overrepresented in individuals with hip osteoarthritis.
    Defects in ASPN are a cause of susceptibility to intervertebral disk disease (IDD) [MIM:603932]. A common musculo-skeletal disorder caused by degeneration of intervertebral disks of the lumbar spine. It results in low-back pain and unilateral leg pain. Note=Susceptibility to intervertebral disk disease, particularly lumbar disk degeneration, is conferred by a triplet repeat expansion polymorphism. ASPN allele having 14 aspartic acid repeats in the N-terminal region of the protein (D14), is associated with the disorder in some populations (PubMed:18304494).
  • 序列相似性
    Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
    Contains 11 LRR (leucine-rich) repeats.
    Contains 1 LRRNT domain.
  • 结构域
    The LRR 5 repeat can inhibit BMP2-induced cytodifferentiation and may be involved in the interaction with BMP2 (By similarity). The repeats LRR 10, LRR 11 and LRR 12 are involved in binding type I collagen. The poly-Asp region is involved in binding calcium.
  • 翻译后修饰
    There is no serine/glycine dipeptide sequence expected for the attachment of O-linked glycosaminoglycans and this is probably not a proteoglycan. The O-linked polysaccharide on 54-Ser is probably the mucin type linked to GalNAc.
    The N-linked glycan at Asn-282 is composed of variable structures of GlcNAc, mannose, fucose, HexNAc and hexose.
  • 细胞定位
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ASPN antibody
    • ASPN protein antibody
    • ASPN_HUMAN antibody
    • Asporin (LRR class 1) antibody
    • Asporin antibody
    • Asporin proteoglycan antibody
    • FLJ20129 antibody
    • LRR class 1 antibody
    • Periodontal ligament associated protein 1 antibody
    • Periodontal ligament-associated protein 1 antibody
    • PLAP 1 antibody
    • PLAP-1 antibody
    • PLAP1 antibody
    • SLRR 1C antibody
    • SLRR1C antibody
    • Small leucine rich protein 1C antibody
    see all

Anti-Asporin antibody 图像

  • Anti-Asporin antibody (ab58741) at 2.5 µg/ml + HepG2 cell lysate at 10 µg with skim milk in PBS buffer at 5 %

    Secondary
    HRP conjugated anti-Rabbit IgG at 1: 50,000 - 100,000

    Predicted band size : 43 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%
  • ICC/IF image of ab58741 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58741, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling Asporin with ab58741 at a concentration of 4-8µg/ml. Skeletal muscle cells (indicated by arrows) were positively stained. Magnification: 400X.

Anti-Asporin antibody (ab58741)参考文献

This product has been referenced in:
  • Wang S  et al. IL-1ß increases asporin expression via the NF-?B p65 pathway in nucleus pulposus cells during intervertebral disc degeneration. Sci Rep 7:4112 (2017). WB, IHC ; Rabbit . Read more (PubMed: 28646230) »
  • Kawamura R  et al. EDTA soluble chemical components and the conditioned medium from mobilized dental pulp stem cells contain an inductive microenvironment, promoting cell proliferation, migration, and odontoblastic differentiation. Stem Cell Res Ther 7:77 (2016). Read more (PubMed: 27387974) »

See all 3 Publications for this product

Product Wall

Thank you for contacting us and sorry for the delay in our response. With this antibody we would recommend that it be aliquotted on delivery (following centrifugation at 10,000 x g for 20 seconds) and stored at -20°C as you say to avoid freeze thaw...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Human Recombinant protein (from abnova Catalog # : H00054829-P01)
Specification
from abnova Catalog # : H00054829-P01
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Type
Direct
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提交于 Oct 06 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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