概述

  • 产品名称Anti-Asporin抗体
    参阅全部 Asporin 一抗
  • 描述
    山羊多克隆抗体to Asporin
  • 经测试应用适用于: WB, IHC-P, ICC/IF, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide:

    C-IHENKVKKIQKDT

    , corresponding to Internal sequence amino acids 182-194 of Human Asporin. (Peptide available as ab134013.)

  • 阳性对照
    • Human bone marrow lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab31303 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa).Can be blocked with Human Asporin peptide (ab134013).
IHC-P Use at an assay dependent dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. PubMed: 20541802
ICC/IF Use at an assay dependent dilution.
ELISA Use at an assay dependent concentration.

靶标

  • 功能Negatively regulates periodontal ligament (PDL) differentiation and mineralization to ensure that the PDL is not ossified and to maintain homeostasis of the tooth-supporting system. Inhibits BMP2-induced cytodifferentiation of PDL cells by preventing its binding to BMPR1B/BMP type-1B receptor, resulting in inhibition of BMP-dependent activation of SMAD proteins (By similarity). Critical regulator of TGF-beta in articular cartilage and plays an essential role in cartilage homeostasis and osteoarthritis (OA) pathogenesis. Negatively regulates chondrogenesis in the articular cartilage by blocking the TGF-beta/receptor interaction on the cell surface and inhibiting the canonical TGF-beta/Smad signal. Binds calcium and plays a role in osteoblast-driven collagen biomineralization activity.
  • 组织特异性Higher levels in osteoarthritic articular cartilage, aorta, uterus. Moderate expression in small intestine, heart, liver, bladder, ovary, stomach, and in the adrenal, thyroid, and mammary glands. Low expression in trachea, bone marrow, and lung. Co-localizes with TGFB1 in chondrocytes within osteoarthritic (OA) lesions of articular cartilage.
  • 疾病相关Genetic variations in ASPN are associated with susceptibility to osteoarthritis type 3 (OS3) [MIM:607850]; also known as osteoarthritis of knee/hip. Osteoarthritis is a degenerative disease of the joints characterized by degradation of the hyaline articular cartilage and remodeling of the subchondral bone with sclerosis. Clinical symptoms include pain and joint stiffness often leading to significant disability and joint replacement. Note=Susceptibility to osteoarthritis is conferred by a triplet repeat expansion polymorphism. ASPN allele having 14 aspartic acid repeats in the N-terminal region of the protein (D14), is overrepresented relative to the common allele having 13 aspartic acid repeats (D13). The frequency of the D14 allele increases with disease severity. The D14 allele is also overrepresented in individuals with hip osteoarthritis.
    Defects in ASPN are a cause of susceptibility to intervertebral disk disease (IDD) [MIM:603932]. A common musculo-skeletal disorder caused by degeneration of intervertebral disks of the lumbar spine. It results in low-back pain and unilateral leg pain. Note=Susceptibility to intervertebral disk disease, particularly lumbar disk degeneration, is conferred by a triplet repeat expansion polymorphism. ASPN allele having 14 aspartic acid repeats in the N-terminal region of the protein (D14), is associated with the disorder in some populations (PubMed:18304494).
  • 序列相似性Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
    Contains 11 LRR (leucine-rich) repeats.
    Contains 1 LRRNT domain.
  • 结构域The LRR 5 repeat can inhibit BMP2-induced cytodifferentiation and may be involved in the interaction with BMP2 (By similarity). The repeats LRR 10, LRR 11 and LRR 12 are involved in binding type I collagen. The poly-Asp region is involved in binding calcium.
  • 翻译后修饰There is no serine/glycine dipeptide sequence expected for the attachment of O-linked glycosaminoglycans and this is probably not a proteoglycan. The O-linked polysaccharide on 54-Ser is probably the mucin type linked to GalNAc.
    The N-linked glycan at Asn-282 is composed of variable structures of GlcNAc, mannose, fucose, HexNAc and hexose.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ASPN antibody
    • ASPN protein antibody
    • ASPN_HUMAN antibody
    • Asporin (LRR class 1) antibody
    • Asporin antibody
    • Asporin proteoglycan antibody
    • FLJ20129 antibody
    • LRR class 1 antibody
    • Periodontal ligament associated protein 1 antibody
    • Periodontal ligament-associated protein 1 antibody
    • PLAP 1 antibody
    • PLAP-1 antibody
    • PLAP1 antibody
    • SLRR 1C antibody
    • SLRR1C antibody
    • Small leucine rich protein 1C antibody
    see all

Anti-Asporin antibody 图像

  • All lanes : Anti-Asporin antibody (ab31303) at 1/1000 dilution

    Lane 1 : Human osteosarcoma cell line whole lysate
    Lane 2 : Human osteosarcoma cell line whole lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    HRP-conjugated anti-goat IgG polyclonal at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    Blocked with 5% milk for 1 hour at 27°C.

    Incubated with the primary antibody diluted in blocking buffer for 12 hours at 4°C.

    See Abreview

  • Anti-Asporin antibody (ab31303) at 0.3 µg/ml + Human Bone Marrow lysate (35µg protein in RIPA buffer)

    Predicted band size : 43 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    Primary incubation was 1 hour. Detected by chemiluminescence.
  • ab31303 (4µg/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Shows textured staining in the cytoplasm of absorptive cells.
  • Ab31303 staining of ATDC5 cells (Panels A and C) and DAPI (panels B and D). Image shows staining of cytoplasm and on the cell surface. Data gratefuly received from Dr. Shiro Ikegawa, SNP Research Center, RIKEN, Japan.

Anti-Asporin antibody (ab31303)参考文献

This product has been referenced in:
  • Hurng JM  et al. Discontinuities in the human bone-PDL-cementum complex. Biomaterials 32:7106-17 (2011). ICC/IF ; Human . Read more (PubMed: 21774982) »
  • Ho SP  et al. The biomechanical characteristics of the bone-periodontal ligament-cementum complex. Biomaterials 31:6635-46 (2010). IHC-P ; Human . Read more (PubMed: 20541802) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Osteosarcoma cell lines)
Gel Running Conditions Reduced Denaturing
Loading amount 30 µg
Specification Osteosarcoma cell lines
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Username

Abcam user community

Verified customer

提交于 Jun 19 2015

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this peptide.

The details you have kindly provided will enable us to investigate this case for you and this is also h...

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Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which gu...

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We will be adding the peptide to our catalogue, and I will inform you as soon as this has been done.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for your enquiry.

I can confirm that this antibody was tested in IHC-P in the publication provided on the datasheet:

Ho SP et al. The biomechanical characteristics of the bone-periodontal ligament-cementum complex. Bioma...

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Thank you for taking the time to contact us.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for WB, ELISA, IHC-P and ICC/IF with human samples. In the event that a product is not functioning in ...

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Thank you for contacting Abcam. Below is a link to our general antigen-retrieval protocol, although the protocol may have to be optimized for each antibody: http://www.abcam.com/index.html?pageconfig=resource&rid=11488 However look...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (primary chondrocytes)
Loading amount 30 µg
Specification primary chondrocytes
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Feb 28 2011

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Recombinant protein (from abnova Catalog # : H00054829-P01)
Specification from abnova Catalog # : H00054829-P01
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Type Direct
Username

Abcam user community

Verified customer

提交于 Oct 06 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"