Aspartate Assay试剂盒(ab102512)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture supernatant, Other biological fluids, Serum, Tissue Extracts, Urine
- Sensitivity: 2 µM
概述
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产品名称
Aspartate Assay试剂盒 -
检测方法
Colorimetric/Fluorometric -
样品类型
Cell culture supernatant, Urine, Serum, Other biological fluids, Tissue Extracts -
检测类型
Quantitative -
灵敏度
> 2 µM -
范围
2 µM - 200 µM -
检测时间
0h 40m -
产品概述
Aspartate Assay Kit ab102512 provides a simple, convenient assay to measure aspartate in a variety of samples.
In the aspartate assay protocol, aspartate is converted to pyruvate which is oxidized with the conversion of a probe into a highly colored (570 nm) and fluorescent (Ex/Em 535/587 nm) species proportional to the amount of aspartate in samples. Aspartate can be quantified in the range between 0.1–10 nmoles/well (2-200 µM).
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说明
This product is manufactured by BioVision, an Abcam company and was previously called K552 Aspartate Colorimetric/Fluorometric Assay Kit. K552-100 is the same size as the 100 test size of ab102512.
L-Aspartic acid (Asp) is one of the 20 proteinogenic amino acids as building block for protein. Although a non-essential amino acid in mammals, it is a precursor to several other amino acids including four essential amino acids (Met, Thr, Ile and Lys).
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests Aspartate Enzyme Mix 1 vial Aspartate Standard 1 x 0.1ml Assay Buffer IV 1 x 25ml Converter Enzyme XII 1 vial OxiRed Probe 1 x 0.2ml Sample Clean-Up Mix I 1 vial -
研究领域
图片
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Aspartate levels measured colourometrically in mouse tissue lysates (mg of extracted protein: background signal subtracted, mean of duplicates; +/- SD).
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Aspartate levels measured fluorometrically in cell lysates (background signal subtracted, mean of duplicates; +/- SD).
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Aspartate levels measured fluorometrically in rat biological fluids, background signal subtracted (duplicates +/- SD).
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Fluorometric Standard Curve Performed as Described in the Protocol
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Colorimetric Standard Curve Performed as Described in the Protocol
数据表及文件
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SDS download
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Datasheet download
文献 (14)
ab102512 被引用在 14 文献中.
- Hickman TL et al. BOXR1030, an anti-GPC3 CAR with exogenous GOT2 expression, shows enhanced T cell metabolism and improved anti-cell line derived tumor xenograft activity. PLoS One 17:e0266980 (2022). PubMed: 35507536
- Naz S et al. Pharmacological Inhibition of HSP90 Radiosensitizes Head and Neck Squamous Cell Carcinoma Xenograft by Inhibition of DNA Damage Repair, Nucleotide Metabolism, and Radiation-Induced Tumor Vasculogenesis. Int J Radiat Oncol Biol Phys 110:1295-1305 (2021). PubMed: 33838214
- Yan S et al. Discovery of GOT1 Inhibitors from a Marine-Derived Aspergillus terreus That Act against Pancreatic Ductal Adenocarcinoma. Mar Drugs 19:N/A (2021). PubMed: 34822459
- Bertero T et al. Tumor-Stroma Mechanics Coordinate Amino Acid Availability to Sustain Tumor Growth and Malignancy. Cell Metab 29:124-140.e10 (2019). PubMed: 30293773
- Wang J et al. miR-9-5p inhibits pancreatic cancer cell proliferation, invasion and glutamine metabolism by targeting GOT1. Biochem Biophys Res Commun 509:241-248 (2019). PubMed: 30591220