Aspartate Aminotransferase Activity Assay试剂盒(ab105135)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 10 mU/well
概述
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产品名称
Aspartate Aminotransferase Activity Assay试剂盒
参阅全部 Aspartate Aminotransferase 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
检测类型
Enzyme activity -
灵敏度
10 mU/well -
检测时间
1h 00m -
产品概述
Aspartate Aminotransferase Activity Assay Kit (AST assay kit) ab105135 provides a rapid, simple, sensitive and reliable assay for AST activity.
In the AST assay protocol, an amino group is transferred from aspartate to alpha-ketoglutarate. The products of this reversible transamination reaction are oxaloacetate and glutamate. The glutamate is detected in a reaction that converts a nearly colorless probe to color (λmax = 450 nm).
The AST assay has a detection limit of 10 mU per well.
AST assay protocol summary:
- add samples and standards to wells
- add reaction mix
- incubate at 37ºC and analyze with microplate reader after 10 mins and then again after 60 mins
Chinese protocol available. See protocols section below.
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说明
This product is manufactured by BioVision, an Abcam company and was previously called K753 Aspartate Aminotransferase (AST or SGOT) Activity Colorimetric Assay Kit. K753-100 is the same size as the 100 test size of ab105135.
Aspartate aminotransferase (AST), also known as Aspartate transaminase and Glutamate-oxaloacetate transaminase (GOT), is a transaminase (EC 2.6.1.1) similar to the more liver specific alanine transaminase (ALT). Although commonly included clinically as part of a diagnostic liver function test, AST has a broader clinical utility since it may also be elevated in diseases affecting other organs, such as the heart or muscles in myocardial infarction, also in acute pancreatitis, acute hemolytic anemia, severe burns, acute renal disease, musculoskeletal diseases and trauma. It catalyzes the reaction: Aspartate + alpha-Ketoglutarate = Oxaloacetate + Glutamate Diagnostically, it is almost always measured in units/liter (U/L).
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests 2000 tests Assay Buffer XIX 1 x 25ml 20 x 25ml AST Enzyme Mix 1 vial 20 vials AST Positive Control 1 vial 20 vials AST Substrate 1 vial 20 vials Developer Solution III 1 vial 20 vials Glutamate Standard 1 x 0.1ml 20 x 0.1ml -
研究领域
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功能
Plays a key role in amino acid metabolism. -
序列相似性
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. -
细胞定位
Cytoplasm. - Information by UniProt
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别名
- AATC_HUMAN
- Aspartate aminotransferase
- Aspartate aminotransferase 1
see all
图片
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AST activity measured in mouse tissue lysates showing activity (mU) per mg of extracted protein (T1 = 2 min; T2 = 60 min).
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AST activity measured in human biological fluids showing activity (mU) per mL of tested sample, (T1 = 2 min; T2 = 60 min) with background signals subtracted.
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Serum aspartate transaminase (AST) levels were measured using ab105135 and alanine transaminase (ALT) levels were measured using ab105134. Both levels were measured at various time periods post Con A injection. Mean values ± SD are shown (n = 4). ∗P < 0.05 and ∗∗P < 0.01.
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AST activity measured in cell lysates (T1 = 2 min; T2 = 60 min; duplicates (+/- SD), background signal subtracted).
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AST activity measured in cell culture medium and control medium, background signal subtracted. T1 = 2 min; T2 = 60 min.
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Standard curve (60 minutes incubation): mean of duplicates (+/- SD) with background reads subtracted.
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Example of Standard Curve and Positive control detection using ab105135.
数据表及文件
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SDS download
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Datasheet download
文献 (64)
ab105135 被引用在 64 文献中.
- Xiao K et al. Potential roles of vitamin D binding protein in attenuating liver injury in sepsis. Mil Med Res 9:4 (2022). PubMed: 35057868
- Farooqi HMU et al. Study of the Anticancer Potential of Plant Extracts Using Liver Tumor Microphysiological System. Life (Basel) 12:N/A (2022). PubMed: 35207423
- Liu G et al. Hepatic RACK1 deficiency protects against fulminant hepatitis through myeloid-derived suppressor cells. Theranostics 12:2248-2265 (2022). PubMed: 35265209
- Tsai SC et al. Anti-Obesity Effect of Nostoc commune Ethanol Extract In Vitro and In Vivo. Nutrients 14:N/A (2022). PubMed: 35267941
- Fielder E et al. Short senolytic or senostatic interventions rescue progression of radiation-induced frailty and premature ageing in mice. Elife 11:N/A (2022). PubMed: 35507395