重组Anti-Asparagine synthetase抗体[EP282Y] (ab40850)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP282Y] to Asparagine synthetase
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Asparagine synthetase抗体[EP282Y]
参阅全部 Asparagine synthetase 一抗 -
描述
兔单克隆抗体[EP282Y] to Asparagine synthetase -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human Asparagine synthetase aa 500-600 (C terminal). The exact sequence is proprietary.
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阳性对照
- K562 cell lysate, human breast carcinoma tissue and human muscle tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP282Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab40850于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/1000 - 1/2000. Detects a band of approximately 60 kDa (predicted molecular weight: 61 kDa).
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说明 |
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WB
1/1000 - 1/2000. Detects a band of approximately 60 kDa (predicted molecular weight: 61 kDa). |
靶标
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通路
Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1. -
序列相似性
Contains 1 asparagine synthetase domain.
Contains 1 glutamine amidotransferase type-2 domain. - Information by UniProt
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数据库链接
- Entrez Gene: 440 Human
- Entrez Gene: 27053 Mouse
- Entrez Gene: 25612 Rat
- Omim: 108370 Human
- SwissProt: P08243 Human
- SwissProt: Q61024 Mouse
- SwissProt: P49088 Rat
- Unigene: 489207 Human
see all -
别名
- asnS antibody
- ASNS_HUMAN antibody
- ASNSD antibody
see all
图片
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Anti-Asparagine synthetase antibody [EP282Y] (ab40850) at 1/2000 dilution + K562 cell lysate at 10 µg
Predicted band size: 61 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab40850 被引用在 6 文献中.
- Nishikawa G et al. Targeting Asparagine Synthetase in Tumorgenicity Using Patient-Derived Tumor-Initiating Cells. Cells 11:N/A (2022). PubMed: 36291140
- Mukherjee A et al. Asparagine Synthetase Is Highly Expressed at Baseline in the Pancreas Through Heightened PERK Signaling. Cell Mol Gastroenterol Hepatol 9:1-13 (2020). PubMed: 31421261
- Restall IJ et al. Brain Tumor Stem Cell Dependence on Glutaminase Reveals a Metabolic Vulnerability through the Amino Acid Deprivation Response Pathway. Cancer Res 80:5478-5490 (2020). PubMed: 33106333
- Huang ML et al. Molecular and functional alterations in a mouse cardiac model of Friedreich ataxia: activation of the integrated stress response, eIF2a phosphorylation, and the induction of downstream targets. Am J Pathol 183:745-57 (2013). WB ; Mouse . PubMed: 23886890
- Scotti C et al. Cell-cycle inhibition by Helicobacter pylori L-asparaginase. PLoS One 5:e13892 (2010). WB, IHC-P ; Human . PubMed: 21085483
- Pos Z et al. Histamine suppresses fibulin-5 and insulin-like growth factor-II receptor expression in melanoma. Cancer Res 68:1997-2005 (2008). PubMed: 18339882