Anti-Asparaginase抗体(Biotin) (ab34616)


  • 产品名称Anti-Asparaginase抗体(Biotin)
    参阅全部 Asparaginase 一抗
  • 描述
    兔多克隆抗体to Asparaginase (Biotin)
  • 偶联物Biotin
  • 经测试应用适用于: Immunomicroscopy, IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Escherichia coli
  • 免疫原

    Asparaginase (Escherichia coli )



Our Abpromise guarantee covers the use of ab34616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Immunomicroscopy Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. PubMed: 45957
WB Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.
ELISA Use at an assay dependent concentration.


  • 相关性Asparaginase is an enzyme purified from E. coli and Erwinia carotovora. It acts by deaminating extracellular L asparagine, an amino acid that appears to be essential for protein synthesis by some tumour cells which are unable to synthesise asparagine. Asparaginase from Erwinia carotovora is serologically and biochemically distinct from asparaginase from E. coli, although its antineoplastic activity and toxicity is similar. Asparaginase is usually considered to be cell cycle phase nonspecific, but it may block some cells in G1 or S phase. Asparaginase reduces cellular and humoral immunity. E.coli contains two L asparaginase isoenzymes: L asparaginase I, a low affinity enzyme located in the cytoplasm, and L asparaginase II, a high affinity secreted enzyme.
  • 细胞定位Cytoplasmic, Secreted and Periplasm.
  • 数据库链接
    • 别名
      • AnsA antibody
      • AnsB antibody
      • Colaspase antibody
      • Cytoplasmic asparaginase I antibody
      • L ASNase I antibody
      • L ASNase II antibody
      • L asparaginase 1 antibody
      • L asparaginase 2 antibody
      • L asparaginase I antibody
      • L asparaginase II antibody
      • L asparaginase II precursor antibody
      • L asparagine amidohydrolase I antibody
      • L asparagine amidohydrolase II antibody
      see all

    Anti-Asparaginase antibody (Biotin) 图像

    • ab34616 staining Asparaginase in Mice treated with E.coli Asparaginase or mock treated mice were sacrificed 16 hours after injection. Tissue was stained for Asparaginase uptake by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 2% Normal Goat serum + 2% BSA for 30 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/1500 in blocking buffer) for 1 hours at 20°C.

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    Anti-Asparaginase antibody (Biotin) (ab34616)参考文献

    ab34616 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step (agent) for 30 minute(s) · Concentration: 2% · Temperature: 20°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Citrate, pH6
    Sample Mouse Tissue sections (Liver)
    Specification Liver
    Permeabilization No
    Fixative Formaldehyde

    Abcam user community

    Verified customer

    提交于 Oct 31 2014

    Thank you for your enquiry. I have contacted the originator of this antibody and unfortunately we do not know which asparaginase this antibody is specific to; we only know that it reacts against asparaginase in E. coli. I am sorry that we do not have m...

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