by a Cysteine residue linker, corresponding to internal sequence amino acids 397-406 of Human ASNSD1 (NP_061921.1).
- HEK293 transfected lysate overexpressing Human ASNSD1.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
Immunogen affinity purified
ab112523 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa). for 1 hour.
Contains 1 asparagine synthetase domain.
Contains 1 glutamine amidotransferase type-2 domain.
Information by UniProt
- ASND1_HUMAN antibody
- ASNSD 1 antibody
- Asnsd1 antibody
Western blot - Anti-ASNSD1 antibody (ab112523)
Lane 1 :
Anti-ASNSD1 antibody (ab112523) at 1 µg/mlLane 2 :
Anti-ASNSD1 antibody (ab112523) at 1 mg/mlLane 3 :
anti-DYKDDDDK Tag at 1/10000 dilutionLanes 1 & 3 :
HEK293 lysate (in RIPA buffer) overexpressing Human ASNSD1 with DYKDDDDK tag at 10 µgLane 2 :
Developed using the ECL technique.Predicted band size:
72 kDaObserved band size:
75 kDa (why is the actual band size different from the predicted?
has not yet been referenced specifically in any publications.
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