概述

  • 产品名称
    Anti-ASK1 (phospho S83)抗体
    参阅全部 ASK1 一抗
  • 描述
    兔多克隆抗体to ASK1 (phospho S83)
  • 特异性
    ab47304 detects endogenous levels of ASK1 only when phosphorylated at serine 83.
  • 经测试应用
    适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    synthesized phosphopeptide derived from human ASK1 around the phosphorylation site of serine 83 (G-S-SP-V-G)

  • 阳性对照
    • Human breast carcinoma tissue and TNF alpha stimulated K562 cell extracts

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    The antibody was purified using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab47304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 155 kDa (predicted molecular weight: 155 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.

靶标

  • 功能
    Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death.
  • 组织特异性
    Abundantly expressed in heart and pancreas.
  • 序列相似性
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    Dephosphorylated and activated by PGAM5.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Apoptosis signal regulating kinase 1 antibody
    • Apoptosis signal-regulating kinase 1 antibody
    • ASK 1 antibody
    • ASK-1 antibody
    • ASK1 antibody
    • M3K5 antibody
    • M3K5_HUMAN antibody
    • MAP/ERK kinase kinase 5 antibody
    • MAP3K5 antibody
    • MAPK/ERK kinase kinase 5 antibody
    • MAPKKK5 antibody
    • MEK kinase 5 antibody
    • MEKK 5 antibody
    • MEKK5 antibody
    • Mitogen activated protein kinase kinase kinase 5 antibody
    • Mitogen-activated protein kinase kinase kinase 5 antibody
    see all

图片

  • ab47304 staining human breast carcinoma tissue by IHC-P (left hand panel). The right hand panel shows staining in the presence of phospho-peptide.
  • All lanes : Anti-ASK1 (phospho S83) antibody (ab47304)

    Lane 1 : TNF alpha stimulated K562 cells
    Lane 2 : K562 cells


    Predicted band size : 155 kDa

文献

This product has been referenced in:
  • Liangpunsakul S  et al. Imipramine blocks ethanol-induced ASMase activation, ceramide generation, and PP2A activation, and ameliorates hepatic steatosis in ethanol-fed mice. Am J Physiol Gastrointest Liver Physiol 302:G515-23 (2012). Read more (PubMed: 22194417) »

See 1 Publication for this product

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