Anti-Asialoglycoprotein Receptor 1抗体(ab42488)

概述

  • 产品名称Anti-Asialoglycoprotein Receptor 1抗体
    参阅全部 Asialoglycoprotein Receptor 1 一抗
  • 描述
    兔多克隆抗体to Asialoglycoprotein Receptor 1
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Dog
  • 免疫原

    Synthetic peptide found within the range of 50 amino acids 99-148 of Human Asialoglycoprotein Receptor 1

  • 阳性对照
    • Human fetal liver lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab42488 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 33 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use at an assay dependent concentration. PubMed: 23908685

靶标

  • 功能Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.
  • 组织特异性Expressed exclusively in hepatic parenchymal cells.
  • 序列相似性Contains 1 C-type lectin domain.
  • 翻译后修饰Phosphorylated on a cytoplasmic Ser residue.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ASGP-R 1 antibody
    • ASGPR 1 antibody
    • ASGPR antibody
    • ASGPR1 antibody
    • Asgr1 antibody
    • ASGR1_HUMAN antibody
    • Asialoglycoprotein receptor 1 antibody
    • C type lectin domain family 4 member H1 antibody
    • C-type lectin domain family 4 member H1 antibody
    • CLEC4H1 antibody
    • Hepatic lectin H1 antibody
    • HL-1 antibody
    see all

Anti-Asialoglycoprotein Receptor 1 antibody 图像

  • ab42488 staining Asialoglycoprotein Receptor 1 (red) in rat hepatocyte cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 1% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/50 in PBS + 1% donkey serum) for 1 hour at 22°C. An undiluted DyLight® 594-conjugated donkey anti-rabbit IgG polyclonal was used as the secondary antibody. Blue - nucleus (DAPI).

    See Abreview

  • Anti-Asialoglycoprotein Receptor 1 antibody (ab42488) at 0.5 µg/ml (in 5% skim milk / PBS buffer) + human fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 33 kDa
    Observed band size : 33 kDa
    Gel concentration: 12%.

Anti-Asialoglycoprotein Receptor 1 antibody (ab42488)参考文献

This product has been referenced in:
  • Nel I  et al. Individual profiling of circulating tumor cell composition and therapeutic outcome in patients with hepatocellular carcinoma. Transl Oncol 6:420-8 (2013). ICC/IF ; Human . Read more (PubMed: 23908685) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HepG2 cells)
Gel Running Conditions Reduced Denaturing (15% SDS-PAGE)
Loading amount 30 µg
Specification HepG2 cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

提交于 Oct 09 2015

Thank you for your inquiry. The immunogen is based off of isoform 1 which is often referred to as isoform H1a.

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Sample Rat Tissue lysate - other (Rat hepatocytes plasma membrane fraction)
Specification Rat hepatocytes plasma membrane fraction
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

提交于 Oct 28 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Sample Rat Cell (Rat hepatocytes)
Specification Rat hepatocytes
Permeabilization No
Fixative Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

提交于 Oct 27 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"