概述

  • 产品名称Anti-ASH2L抗体
    参阅全部 ASH2L 一抗
  • 描述
    兔多克隆抗体to ASH2L
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within the internal amino acids 288-337 (AAGSSGKGRG AKRKQQDGGT TGTTKKARSD PLFSAQRLPP HGYPLEHPFN) of Human ASH2L (NP_004665).

  • 阳性对照
    • HeLa cell lysate.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab84732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. May function as a transcriptional regulator. May play a role in hematopoiesis.
  • 组织特异性Ubiquitously expressed. Predominantly expressed in adult heart and testis and fetal lung and liver, with barely detectable expression in adult lung, liver, kidney, prostate, and peripheral leukocytes.
  • 序列相似性Contains 1 B30.2/SPRY domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ASH 2 antibody
    • Ash2 (absent small or homeotic) like antibody
    • Ash2 (absent, small, or homeotic) like (Drosophila) antibody
    • ASH2 antibody
    • ASH2 LIKE antibody
    • ASH2 like protein antibody
    • ASH2-like protein antibody
    • Ash2l antibody
    • ASH2L_HUMAN antibody
    • ASH2L1 antibody
    • ASH2L2 antibody
    • Bre 2 antibody
    • Bre2 antibody
    • Discs 2-like antibody
    • Drosophila absent, small, or homeotic antibody
    • Drosophila ASH2-like antibody
    • Set1/Ash2 histone methyltransferase complex subunit ASH2 antibody
    see all

Anti-ASH2L antibody 图像

  • Anti-ASH2L antibody (ab84732) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 69 kDa
    Observed band size : 69 kDa
    Additional bands at : approx. 75 kDa. We are unsure as to the identity of these extra bands.
    12% gel
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling ASH2HL with ab84732 at 5.0µg/ml.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling ASH2L with ab84732 at 5.0µg/ml.

Anti-ASH2L antibody (ab84732)参考文献

ab84732 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab84732.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"