Anti-ASF1b抗体(ab70126)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to ASF1b
  • 特异性
    ab70126 detects endogenous levels of total ASF1B protein.
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthesized peptide derived from internal of human ASF1B.

  • 阳性对照
    • Extracts from K562 cells

性能

应用

Our Abpromise guarantee covers the use of ab70126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 22 kDa.
ELISA 1/5000.

靶标

  • 功能
    Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly. Does not participate in replication-independent nucleosome deposition which is mediated by ASF1A and HIRA. Required for spermatogenesis.
  • 组织特异性
    Highly expressed in testis and at lower levels in colon, small intestine and thymus.
  • 序列相似性
    Belongs to the ASF1 family.
  • 翻译后修饰
    Phosphorylated by TLK1 and TLK2.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Anti silencing function 1B antibody
    • Anti silencing function 1B histone chaperone antibody
    • Anti-silencing function protein 1 homolog B antibody
    • ASF1 anti silencing function 1 homolog B (S. cerevisiae) antibody
    • ASF1 anti silencing function 1 homolog B antibody
    • ASF1B antibody
    • ASF1B_HUMAN antibody
    • CCG1 interacting factor A II antibody
    • CCG1-interacting factor A-II antibody
    • CIA-II antibody
    • hAsf1 antibody
    • hAsf1b antibody
    • hCIA-II antibody
    • Histone chaperone ASF1B antibody
    see all

图片

  • All lanes : Anti-ASF1b antibody (ab70126) at 1/500 dilution

    Lane 1 : Extracts from K562 cells
    Lane 2 : Extracts from K562 cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 22 kDa
    Observed band size : 26 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab70126 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton X100
Username

Dr. Kirk Mcmanus

Verified customer

提交于 Mar 14 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册