概述

  • 产品名称
    Anti-ASCL4抗体
  • 描述
    兔多克隆抗体to ASCL4
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (MMETRKPAER LALPYSLRTA PLGVPGTLPG LPRRDPLRVA LRLDAACWEW) of Human ASCL4 (NP_982260).

  • 阳性对照
    • MCF7 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab81150 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1/312500.

WB Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 相关性
    ASCL4 is a basic helix-loop-helix transcription factor. It is essential for the determination of cell fate and the development and differentiation of numerous tissues. It could be a transcriptional regulator involved in skin development.
  • 细胞定位
    Nucleus
  • 数据库链接
  • 别名
    • Achaete scute complex homolog 4 (Drosophila) antibody
    • Achaete scute complex like 4 antibody
    • Achaete scute like protein 4 antibody
    • bHLHa44 antibody
    • Class II bHLH protein ASCL4 antibody
    • HASH4 antibody
    see all

图片

  • Anti-ASCL4 antibody (ab81150) at 1 µg/ml + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 19 kDa
    Observed band size : 19 kDa

实验方案

文献

ab81150 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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