概述

  • 产品名称
  • 描述
    兔多克隆抗体to Ascl2
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Ascl2.

  • 阳性对照
    • This antibody gave a positive signal in Human Placenta tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab116699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 20 kDa). Abcam recommends using milk as the blocking agent (3%).

靶标

图片

  • Anti-Ascl2 antibody (ab116699) at 1 µg/ml (Milk blocking - 3%) + Human placenta tissue lysate - total protein (ab29745) at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 20 kDa
    Observed band size: 22 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 12 minutes


    This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab116699 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

实验方案

文献

ab116699 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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