概述

  • 产品名称
  • 描述
    兔多克隆抗体to ASC1
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide, corresponding to amino acids 225-275 of Human ASC1 (NP_057297.2)

  • 阳性对照
    • HeLa whole cell lysate 293T whole cell lysate NIH3T3 whole cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab70627 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能
    Transcription coactivator of nuclear receptors which functions in conjunction with CBP-p300 and SRC-1 and may play an important role in establishing distinct coactivator complexes under different cellular conditions. Plays a pivotal role in the transactivation of NF-kappa-B, SRF and AP1. Acts as a mediator of transrepression between nuclear receptor and either AP1 or NF-kappa-B. Plays a role in androgen receptor transactivation and in testicular function.
  • 细胞定位
    Nucleus. Cytoplasm. Cytoplasmic under conditions of serum deprivation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Activating signal cointegrator 1 antibody
    • ASC 1 antibody
    • ASC-1 antibody
    • HsT17391 antibody
    • Thyroid hormone receptor interactor 4 antibody
    • Thyroid receptor interacting protein 4 antibody
    • Thyroid receptor-interacting protein 4 antibody
    • TR-interacting protein 4 antibody
    • TRIP 4 antibody
    • TRIP-4 antibody
    • Trip4 antibody
    • TRIP4_HUMAN antibody
    • ZC2HC5 antibody
    • Zinc finger C2HC5 type antibody
    see all

图片

  • All lanes : Anti-ASC1 antibody (ab70627) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg


    Predicted band size : 66 kDa
    Observed band size : 66 kDa


    Exposure time : 10 seconds
  • Immunoprecipitation of HeLa whole cell lysate (1mg) using ab70627 at 3 µg/ml (lane 1) or control IgG (lane 2). 20% of the immunoprecipitate was used for Western blotting, analysed using ab70627 at 1µg/ml and detected by chemiluminescence for 10 seconds.

文献

This product has been referenced in:
  • Thompson M  et al. Paradoxical roles of serine racemase and D-serine in the G93A mSOD1 mouse model of amyotrophic lateral sclerosis. J Neurochem 120:598-610 (2012). Read more (PubMed: 22117694) »

See 1 Publication for this product

客户评价及客户问答

Application
Western blot
Sample
Human Cell lysate - whole cell (human hepatocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
human hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Jan 27 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Hela Cells)
Loading amount
20 µg
Specification
Hela Cells
Gel Running Conditions
Non-reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Jun 16 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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