Recombinant protein fragment, corresponding to a region within amino acids 1-253 of Human ASB9 (NP_001026909).
HeLaS3, HepG2 and MOLT4 cell lysates for WB; HeLa cells for IF/ICC
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 32 kDa.
1/100 - 1/200.
ASB9 may be a substrate-recognition component of an SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
Ankyrin repeat and SOCS box protein 9 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASB9 antibody (ab97918)
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ab97918 antibody at 1:500 dilution.
Western blot - ASB9 antibody (ab97918)
All lanes : Anti-ASB9 antibody (ab97918) at 1/1000 dilution Lane 1 : HeLaS3 whole cell lysate Lane 2 : HepG2 whole cell lysate Lane 3 : MOLT4 whole cell lysate Lysates/proteins at 30 µg per lane. Predicted band size : 32 kDa 12% SDS PAGE
Immunocytochemistry/ Immunofluorescence - ASB9 antibody (ab97918)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab97918 at 1/200 dilution. Lower image merged with DNA probe.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"