概述

  • 产品名称Anti-ASB6抗体
    参阅全部 ASB6 一抗
  • 描述
    小鼠多克隆抗体to ASB6
  • 特异性Antibody reactive against mammalian transfected lysate.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein, corresponding to amino acids 1-421 of Human ASB6

  • 阳性对照
    • Transfected 293T cell lysate and human liver lysate

性能

应用

Our Abpromise guarantee covers the use of ab67768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: 1/500 - 1/1000. Predicted molecular weight: 47 kDa.
    Detection pair

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Probable substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
    • 通路Protein modification; protein ubiquitination.
    • 序列相似性Contains 6 ANK repeats.
      Contains 1 SOCS box domain.
    • 结构域The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin-protein ligase complexes.
    • 细胞定位Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Ankyrin repeat and SOCS box containing 6 antibody
      • Ankyrin repeat and SOCS box protein 6 antibody
      • ASB 6 antibody
      • ASB-6 antibody
      • ASB6 antibody
      • ASB6_HUMAN antibody
      • FLJ20548 antibody
      • MGC1024 antibody
      see all

    Anti-ASB6 antibody 图像

    • Anti-ASB6 antibody (ab67768) at 1/500 dilution + ASB6 expression in human liver at 25 µg

      Secondary
      Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

      Predicted band size : 47 kDa
      Observed band size : 52 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-ASB6 antibody (ab67768) at 1/500 dilution

      Lane 1 : ASB6 transfected lysate
      Lane 2 : Non transfected lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

      Predicted band size : 47 kDa
      Observed band size : 40 kDa (why is the actual band size different from the predicted?)

    Anti-ASB6 antibody (ab67768)参考文献

    ab67768 has not yet been referenced specifically in any publications.

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