概述

  • 产品名称Anti-ASAH2抗体
    参阅全部 ASAH2 一抗
  • 描述
    兔多克隆抗体to ASAH2
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide surrounding amino acid 741 of human ASAH2

  • 阳性对照
    • Jurkat cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 30% Glycerol, 0.5% BSA, PBS, pH 7.2
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab63804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).

靶标

  • 功能Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract.
  • 组织特异性Primarily expressed in the intestine (PubMed:17334805). Ubiquitously expressed with higher levels in kidney, skeletal muscle and heart (PubMed:10781606). According to PubMed:17334805, ubiquitous expression attributed to ASAH2 may be actually that of the paralog ASAH2B.
  • 序列相似性Belongs to the neutral ceramidase family.
  • 翻译后修饰N-glycosylated. Required for enzyme activity.
    O-glycosylated. Required to retain it as a type II membrane protein at the cell surface.
    Phosphorylated. May prevent ubiquitination and subsequent degradation.
    Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is triggered by nitric oxid.
  • 细胞定位Cell membrane. The neutral ceramidase soluble form is a secreted protein. According to PubMed:10781606, it is mitochondrial. However, they used a shorter form in its N-terminus, which may explain this localization which probably does not exist in vivo.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Acylsphingosine deacylase 2 antibody
    • AI585898 antibody
    • ASAH2 antibody
    • ASAH2_HUMAN antibody
    • BCDase antibody
    • hCD antibody
    • HNAC1 antibody
    • LCDase antibody
    • MGC129777 antibody
    • mitochondrial ceramidase antibody
    • N acylsphingosine amidohydrolase (non lysosomal ceramidase) 2 antibody
    • N acylsphingosine amidohydrolase 2 antibody
    • N CDase antibody
    • N-acylsphingosine amidohydrolase 2 antibody
    • N-CDase antibody
    • NCDase antibody
    • Neutral ceramidase soluble form antibody
    • Non lysosomal ceramidase antibody
    • Non-lysosomal ceramidase antibody
    see all

Anti-ASAH2 antibody 图像

  • All lanes : Anti-ASAH2 antibody (ab63804) at 4 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate


    Predicted band size : 86 kDa
    Observed band size : 86 kDa
    Additional bands at : 68 kDa. We are unsure as to the identity of these extra bands.

Anti-ASAH2 antibody (ab63804)参考文献

ab63804 has not yet been referenced specifically in any publications.

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