概述

  • 产品名称Anti-ASAH2抗体
    参阅全部 ASAH2 一抗
  • 描述
    兔多克隆抗体to ASAH2
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide containing 17 residues from the C-terminal region of Human ASAH2.

  • 阳性对照
    • Human small intestine tissue; 293 cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab113369 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 86 kDa.
IHC-P Use a concentration of 5 µg/ml.

靶标

  • 功能Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract.
  • 组织特异性Primarily expressed in the intestine (PubMed:17334805). Ubiquitously expressed with higher levels in kidney, skeletal muscle and heart (PubMed:10781606). According to PubMed:17334805, ubiquitous expression attributed to ASAH2 may be actually that of the paralog ASAH2B.
  • 序列相似性Belongs to the neutral ceramidase family.
  • 翻译后修饰N-glycosylated. Required for enzyme activity.
    O-glycosylated. Required to retain it as a type II membrane protein at the cell surface.
    Phosphorylated. May prevent ubiquitination and subsequent degradation.
    Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is triggered by nitric oxid.
  • 细胞定位Cell membrane. The neutral ceramidase soluble form is a secreted protein. According to PubMed:10781606, it is mitochondrial. However, they used a shorter form in its N-terminus, which may explain this localization which probably does not exist in vivo.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Acylsphingosine deacylase 2 antibody
    • AI585898 antibody
    • ASAH2 antibody
    • ASAH2_HUMAN antibody
    • BCDase antibody
    • hCD antibody
    • HNAC1 antibody
    • LCDase antibody
    • MGC129777 antibody
    • mitochondrial ceramidase antibody
    • N acylsphingosine amidohydrolase (non lysosomal ceramidase) 2 antibody
    • N acylsphingosine amidohydrolase 2 antibody
    • N CDase antibody
    • N-acylsphingosine amidohydrolase 2 antibody
    • N-CDase antibody
    • NCDase antibody
    • Neutral ceramidase soluble form antibody
    • Non lysosomal ceramidase antibody
    • Non-lysosomal ceramidase antibody
    see all

Anti-ASAH2 antibody 图像

  • Lane 1 : Anti-ASAH2 antibody (ab113369) at 1 µg/ml
    Lane 2 : Anti-ASAH2 antibody (ab113369) at 2 µg/ml

    Lane 1 : 293 cell lysate
    Lane 2 : 293 cell lysate


    Predicted band size : 86 kDa
  • ab113369 at 5ug/ml staining ASAH2 in formalin-fixed paraffin-embedded Human small intestine by immunohistochemistry.

Anti-ASAH2 antibody (ab113369)参考文献

ab113369 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab113369.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"