Anti-ASAH1抗体(ab54822)

概述

  • 产品名称Anti-ASAH1抗体
    参阅全部 ASAH1 一抗
  • 描述
    小鼠单克隆抗体to ASAH1
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: PPWTEDCRKS TYPPSGPTYR GAVPWYTINL DLPPYKRWHE LMLDKAPMLK VIVNSLKNMI NTFVPSGKVM QVVDEKLPGL LGNFPGPFEE EMKGIAAVTD , corresponding to amino acids 25-125 of Human ASAH1

性能

应用

Our Abpromise guarantee covers the use of ab54822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
WB
  • 应用说明WB: Use at a concentration of 1-5 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid.
    • 组织特异性Broadly expressed with highest expression in heart.
    • 疾病相关Defects in ASAH1 are the cause of Farber lipogranulomatosis (FL) [MIM:228000]; also known as Farber disease (FD). This sphingolipid disease is characterized by subcutaneous lipid-loaded nodules, excruciating pain in the joints and extremities, marked accumulation of ceramide in lysosomes, and death by three years of age.
    • 序列相似性Belongs to the acid ceramidase family.
    • 细胞定位Lysosome.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AC antibody
      • ACDase antibody
      • Acid CDase antibody
      • Acid ceramidase antibody
      • Acid ceramidase precursor antibody
      • Acid ceramidase subunit beta antibody
      • Acylsphingosine deacylase antibody
      • ASAH 1 antibody
      • ASAH antibody
      • ASAH1 antibody
      • ASAH1_HUMAN antibody
      • FLJ21558 antibody
      • FLJ22079 antibody
      • N acylsphingosine amidohydrolase (acid ceramidase) 1 antibody
      • N acylsphingosine amidohydrolase 1 antibody
      • N acylsphingosine amidohydrolase antibody
      • N-acylsphingosine amidohydrolase antibody
      • N-acylsphingosine deacylase antibody
      • PHP antibody
      • PHP32 antibody
      • Putative 32 kDa heart protein antibody
      • SMAPME antibody
      see all

    Anti-ASAH1 antibody 图像

    • ASAH1 antibody (ab54822) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human heart.
    • Western blot against tagged recombinant protein immunogen using ab54822 ASAH1 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa

    Anti-ASAH1 antibody (ab54822)参考文献

    ab54822 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (PPC1 Cells (Prostate cancer line))
    Loading amount 35 µg
    Specification PPC1 Cells (Prostate cancer line)
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    提交于 Mar 27 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"