Anti-Arylsulfatase J抗体(ab122344)

概述

  • 产品名称
    Anti-Arylsulfatase J抗体
    参阅全部 Arylsulfatase J 一抗
  • 描述
    兔多克隆抗体to Arylsulfatase J
  • 经测试应用
    适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence, corresponding to amino acids 213-307 (DSPGMCGYDL YENDNAAWDY DNGIYSTQMY TQRVQQILAS HNPTKPIFLY IAYQAVHSPL QAPGRYFEHY RSIININRRR YAAMLSCLDE AINNV) of Human Arylsulfatase J.

  • 阳性对照
    • Human smooth muscle tissue; Human tonsil lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab122344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

靶标

  • 序列相似性
    Belongs to the sulfatase family.
  • 翻译后修饰
    The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Arsj antibody
    • ARSJ_HUMAN antibody
    • arylsulfatase family, member J antibody
    • Arylsulfatase J antibody
    • ASJ antibody
    • FLJ23548 antibody
    • UNQ372/PRO708 antibody
    see all

图片

  • ab122344, at a 1/25 dilution, staining Arylsulfatase J in paraffin-embedded Human smooth muscle tissue by immunohistochemistry.
  • All lanes : Anti-Arylsulfatase J antibody (ab122344) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human plasma
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique

文献

ab122344 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Loading amount
0.02 µg
Gel Running Conditions
Reduced Denaturing (15% SDS PAGE)
Sample
Human Recombinant protein (HEK293 cells)
Specification
HEK293 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Ms. Elena Wiegmann

Verified customer

提交于 Nov 11 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册