Anti-Arylsulfatase D抗体(ab81978)

概述

  • 产品名称
    Anti-Arylsulfatase D抗体
    参阅全部 Arylsulfatase D 一抗
  • 描述
    山羊多克隆抗体to Arylsulfatase D
  • 特异性
    ab81978 is expected to recognise both reported isoforms (NP_001660.2; NP_033667.2).
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    C-DASNGYRALQWNA

    , corresponding to internal sequence amino acids 108-120 of Human Arylsulfatase D (NP_001660.2; NP_033667.2)

  • 阳性对照
    • A459 and HepG2 cell lysates.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 0.5mg/ml Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab81978 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab81978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明
    Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/16,000.
    WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 组织特异性
      Expressed in the pancreas, kidney, liver, lung, placenta, brain and heart.
    • 序列相似性
      Belongs to the sulfatase family.
    • 翻译后修饰
      The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.
    • 细胞定位
      Lysosome.
    • Information by UniProt
    • 数据库链接
    • 别名
      • arsD antibody
      • ARSD_HUMAN antibody
      • Arylsulfatase D antibody
      • ASD antibody
      • EC 3.1.6 antibody
      • OTTHUMP00000022848 antibody
      • OTTHUMP00000022849 antibody
      see all

    图片

    • All lanes : Anti-Arylsulfatase D antibody (ab81978) at 0.3 µg/ml

      Lane 1 : A459 lysate in RIPA buffer
      Lane 2 : A459 lysate in RIPA buffer with immunising peptide

      Lysates/proteins at 35 µg per lane.


      Predicted band size : 65 kDa
      Observed band size : 65 kDa
      Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.

    实验方案

    文献

    ab81978 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    There are currently no Customer reviews or Questions for ab81978.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册