Anti-Arylsulfatase D抗体(ab81978)

概述

  • 产品名称Anti-Arylsulfatase D抗体
    参阅全部 Arylsulfatase D 一抗
  • 描述
    山羊多克隆抗体to Arylsulfatase D
  • 特异性ab81978 is expected to recognise both reported isoforms (NP_001660.2; NP_033667.2).
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    C-DASNGYRALQWNA

    , corresponding to internal sequence amino acids 108-120 of Human Arylsulfatase D (NP_001660.2; NP_033667.2)

  • 阳性对照
    • A459 and HepG2 cell lysates.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 0.5mg/ml Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab81978 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab81978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/16,000.
    WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 组织特异性Expressed in the pancreas, kidney, liver, lung, placenta, brain and heart.
    • 序列相似性Belongs to the sulfatase family.
    • 翻译后修饰The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.
    • 细胞定位Lysosome.
    • Information by UniProt
    • 数据库链接
    • 别名
      • arsD antibody
      • ARSD_HUMAN antibody
      • Arylsulfatase D antibody
      • ASD antibody
      • EC 3.1.6 antibody
      • OTTHUMP00000022848 antibody
      • OTTHUMP00000022849 antibody
      see all

    Anti-Arylsulfatase D antibody 图像

    • All lanes : Anti-Arylsulfatase D antibody (ab81978) at 0.3 µg/ml

      Lane 1 : A459 lysate in RIPA buffer
      Lane 2 : A459 lysate in RIPA buffer with immunising peptide

      Lysates/proteins at 35 µg per lane.


      Predicted band size : 65 kDa
      Observed band size : 65 kDa
      Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.

    Anti-Arylsulfatase D antibody (ab81978)参考文献

    ab81978 has not yet been referenced specifically in any publications.

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