Anti-Aryl hydrocarbon Receptor抗体(ab84833)

概述

  • 产品名称Anti-Aryl hydrocarbon Receptor抗体
    参阅全部 Aryl hydrocarbon Receptor 一抗
  • 描述
    兔多克隆抗体to Aryl hydrocarbon Receptor
  • 经测试应用适用于: Flow Cyt, IHC-Fr, EMSA, ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide from the N terminal residues of human Aryl hydrocarbon Receptor (NM_001621).

  • 阳性对照
    • HepG2 cell lysate. Rat kidney.

性能

  • 形式Lyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, 0.1M Tris glycine, pH 7.2
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab84833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/1 - 1/100. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
EMSA Use at an assay dependent concentration. PubMed: 22287322
ICC/IF 1/1 - 1/100.
WB 1/200 - 1/500. Predicted molecular weight: 96 kDa.
IHC-P 1/100 - 1/500.

靶标

  • 功能Ligand-activated transcriptional activator. Binds to the XRE promoter region of genes it activates. Activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons. Involved in cell-cycle regulation. Likely to play an important role in the development and maturation of many tissues.
  • 组织特异性Expressed in all tissues tested including blood, brain, heart, kidney, liver, lung, pancreas and skeletal muscle.
  • 序列相似性Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.
  • 细胞定位Cytoplasm. Nucleus. Initially cytoplasmic; upon binding with ligand and interaction with a HSP90, it translocates to the nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Ah receptor antibody
    • AhR antibody
    • AHR_HUMAN antibody
    • Aromatic hydrocarbon receptor antibody
    • Aryl hydrocarbon receptor antibody
    • Aryl hydrocarbon receptor precursor antibody
    • bHLHe76 antibody
    • Class E basic helix loop helix protein 76 antibody
    • Class E basic helix-loop-helix protein 76 antibody
    • HGNC:348 antibody
    see all

Anti-Aryl hydrocarbon Receptor antibody 图像

  • Anti-Aryl hydrocarbon Receptor antibody (ab84833) at 1/200 dilution + HepG2 cell lysate

    Predicted band size : 96 kDa
    Observed band size : 96 kDa
  • ab84833, at 1/200 dilution, staining Aryl hydrocarbon Receptor in formalin-fixed paraffin-embedded rat kidney by Immunohistochemistry.
  • Flow cytometry using Aryl hydrocarbon Receptor antibody, ab84833. The histogram above shows ab84833 positive human embryonic stem cells (in green) and secondary only negative control (in purple).

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  • ab84833 staining Aryl hydrocarbon receptor in MDA-MB-231 cells treated with tranilast (ab120643), by ICC/IF. Increase in Aryl hydrocarbon receptor expression correlates with increased concentration of tranilast, as described in literature.
    The cells were incubated at 37°C for 24h in media containing different concentrations of ab120643 (telmisartan) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab84833 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • ab84833 staining Aryl hydrocarbon Receptor in Human colon tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 10% sreum for 30 minutes at 21°C. Samples were incubated with primary antibody for 16 hours at 4°C. An undiluted Alexa fluor®555-conjugated Donkey anti-rabbit IgG polyclonal was used as the secondary antibody.

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  • ab84833 staining Aryl hydrocarbon receptor protein on mouse embryonic stem cell by ICC/IF. The cells were paraformaldehyde fixed, permeabilized in 0.1% triton-x and blocked in 1% serum for 30 minutes at 21°C. The primary antibody was diluted 1/100 and incubated with sample for 2 hours at 21°C. An Alexa Fluor® 488 conjugated goat anti-rabbit IgG, diluted 1/100 was used as secondary.

    See Abreview

Anti-Aryl hydrocarbon Receptor antibody (ab84833)参考文献

This product has been referenced in:
  • Ohno M  et al. Sudan III dye strongly induces CYP1A1 mRNA expression in HepG2 cells. J Biochem Mol Toxicol 26:16-22 (2012). EMSA ; Human . Read more (PubMed: 22287322) »
  • Kretzschmar G  et al. Estradiol regulates aryl hydrocarbon receptor expression in the rat uterus. Mol Cell Endocrinol 321:253-7 (2010). IHC-P . Read more (PubMed: 20176079) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (colon)
Specification colon
Fixative Acetone
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 21°C
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Abcam user community

Verified customer

提交于 Mar 01 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (human colon lysate)
Loading amount 40 µg
Specification human colon lysate
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
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Abcam user community

Verified customer

提交于 Feb 09 2012

Thank you for your inquiry. I can confirm that the flow cytometry image with ab84833 is courtesy of an anonymous Abreview. I would like to suggest to review the Abreview: http://www.abcam.com/index.html?datasheet=84833&tab=abreviews&intabre...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (embryonic stem cell)
Specification embryonic stem cell
Fixative Paraformaldehyde
Permeabilization Yes - 0.1 % triton-x
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Nov 24 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (embryonic stem cell)
Specification embryonic stem cell
Preparation Cell harvesting/tissue preparation method: cell dissociation buffer
Sample buffer: enzyme free
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy cells
Username

Abcam user community

Verified customer

提交于 Nov 23 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"