Anti-Aryl hydrocarbon Receptor抗体(ab28698)

概述

  • 产品名称
    Anti-Aryl hydrocarbon Receptor抗体
    参阅全部 Aryl hydrocarbon Receptor 一抗
  • 描述
    兔多克隆抗体to Aryl hydrocarbon Receptor
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Dog
  • 免疫原

    A region within a synthetic peptide: MNSSSANITY ASRKRRKPVQ KTVKPIPAEG IKSNPSKRHR DRLNTELDRL, corresponding to N terminal amino acids 1-50 of Human Aryl hydrocarbon Receptor

  • 阳性对照
    • Human placenta.

性能

应用

Our Abpromise guarantee covers the use of ab28698 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 85 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    Ligand-activated transcriptional activator. Binds to the XRE promoter region of genes it activates. Activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons. Involved in cell-cycle regulation. Likely to play an important role in the development and maturation of many tissues.
  • 组织特异性
    Expressed in all tissues tested including blood, brain, heart, kidney, liver, lung, pancreas and skeletal muscle.
  • 序列相似性
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.
  • 细胞定位
    Cytoplasm. Nucleus. Initially cytoplasmic; upon binding with ligand and interaction with a HSP90, it translocates to the nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Ah receptor antibody
    • AhR antibody
    • AHR_HUMAN antibody
    • Aromatic hydrocarbon receptor antibody
    • Aryl hydrocarbon receptor antibody
    • Aryl hydrocarbon receptor precursor antibody
    • bHLHe76 antibody
    • Class E basic helix loop helix protein 76 antibody
    • Class E basic helix-loop-helix protein 76 antibody
    • HGNC:348 antibody
    see all

图片

  • Anti-Aryl hydrocarbon Receptor antibody (ab28698) at 2.5 µg/ml + Human placental lysate

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Observed band size : 85 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab28698 has not yet been referenced specifically in any publications.

客户评价及客户问答

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Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Mouse Cell (embryonic stem cell)
Specification
embryonic stem cell
Preparation
Cell harvesting/tissue preparation method: cell dissociation buffer
Sample buffer: enzyme-free
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
cells
Username

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Verified customer

提交于 Dec 17 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (embryonic stem cell)
Loading amount
200000 cells
Specification
embryonic stem cell
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
Username

Abcam user community

Verified customer

提交于 Dec 14 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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