概述

  • 产品名称
    Anti-ARTS1抗体[EPR6069]
    参阅全部 ARTS1 一抗
  • 描述
    兔单克隆抗体[EPR6069] to ARTS1
  • 经测试应用
    适用于: WB, IHC-P, ICC, Flow Cytmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to C-terminal amino acids of Human ARTS1.

  • 阳性对照
    • WB: K562, HUVEC and A431 cell lysates. IHC-P: Human uterus tissue. Flow Cyt: K562 cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab124669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 107 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC 1/250 - 1/500.
Flow Cyt 1/200.
  • 应用说明
    Is unsuitable for IP.
  • 靶标

    • 功能
      Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney.
    • 组织特异性
      Ubiquitous.
    • 序列相似性
      Belongs to the peptidase M1 family.
    • 翻译后修饰
      N-glycosylated.
    • 细胞定位
      Endoplasmic reticulum membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • A LAP antibody
      • A-LAP antibody
      • Adipocyte derived leucine aminopeptidase antibody
      • Adipocyte-derived leucine aminopeptidase antibody
      • ALAP antibody
      • Aminopeptidase PILS antibody
      • Aminopeptidase regulator of TNFR1 shedding antibody
      • APPILS antibody
      • ARTS 1 antibody
      • ARTS-1 antibody
      • Arts1 antibody
      • Endoplasmic reticulum aminopeptidase 1 antibody
      • Endoplasmic reticulum aminopeptidase associated with antigen processing antibody
      • ERAAP antibody
      • ERAAP1 antibody
      • ERAP 1 antibody
      • Erap1 antibody
      • ERAP1_HUMAN antibody
      • KIAA0525 antibody
      • kiaa0525 protein antibody
      • PILS AP antibody
      • PILS-AP antibody
      • PILSA antibody
      • PILSAP antibody
      • Puromycin insensitive leucyl specific aminopeptidase antibody
      • Puromycin-insensitive leucyl-specific aminopeptidase antibody
      • Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator antibody
      • type 1 tumor necrosis factor receptors shedding aminopeptidase regulator antibody
      • VEGF-induced aminopeptidase antibody
      see all

    图片

    • All lanes : Anti-ARTS1 antibody [EPR6069] (ab124669) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HUVEC cell lysate
      Lane 3 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 2000

      Predicted band size : 107 kDa
    • ab124669, at a 1/50 dilution, staining ARTS1 in paraffin embedded Human uterus tissue by Immunohistochemistry.
    • Flow cytometry analysis of K562 (human chronic myelogenous leukemia) cells labeling ARTS1 (red) with ab124669 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

    文献

    This product has been referenced in:
    • Wang B  et al. p53 increases MHC class I expression by upregulating the endoplasmic reticulum aminopeptidase ERAP1. Nat Commun 4:2359 (2013). WB . Read more (PubMed: 23965983) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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