重组Anti-ARTS1/ERAP1抗体[EPR6069] (ab124669)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6069] to ARTS1/ERAP1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
-
产品名称
Anti-ARTS1/ERAP1抗体[EPR6069]
参阅全部 ARTS1/ERAP1 一抗 -
描述
兔单克隆抗体[EPR6069] to ARTS1/ERAP1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human ARTS1/ERAP1 aa 900-1000 (C terminal).
-
阳性对照
- WB: K562, HUVEC, mouse heart, rat spleen and A431 cell lysates. IHC-P: Human uterus and human bladder carcinoma tissue. Flow Cyt: K562 cells.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6069 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Conjugation kits
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124669于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 107 kDa.
|
|
IHC-P |
1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. For unpurified use at 1/50 - 1/100.
|
说明 |
---|
WB
1/1000 - 1/10000. Predicted molecular weight: 107 kDa. |
IHC-P
1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. For unpurified use at 1/50 - 1/100.
|
靶标
-
功能
Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the peptidase M1 family. -
翻译后修饰
N-glycosylated. -
细胞定位
Endoplasmic reticulum membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 51752 Human
- Entrez Gene: 80898 Mouse
- Entrez Gene: 80897 Rat
- Omim: 606832 Human
- SwissProt: Q9NZ08 Human
- SwissProt: Q9EQH2 Mouse
- SwissProt: Q9JJ22 Rat
- Unigene: 716426 Human
see all -
别名
- A LAP antibody
- A-LAP antibody
- Adipocyte derived leucine aminopeptidase antibody
see all
图片
-
Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669) at 1/5000 dilution (purified) + K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 107 kDaBlocking and diluting buffer: 5% NFDM/TBST.
-
All lanes : Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669) at 1/5000 dilution (purified)
Lane 1 : Mouse heart lysates
Lane 2 : Rat spleen lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 107 kDaBlocking and diluting buffer: 5% NFDM/TBST.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder carcinoma tissue sections labeling ARTS1/ERAP1 with Purified ab124669 at 1:1000 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
-
All lanes : Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669) at 1/1000 dilution (unpurified)
Lane 1 : K562 cell lysate
Lane 2 : HUVEC cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP
Predicted band size: 107 kDa -
Unpurified ab124669, at a 1/50 dilution, staining ARTS1/ERAP1 in paraffin embedded Human uterus tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (3)
ab124669 被引用在 3 文献中.
- Liang YH et al. Chemotherapy agents stimulate dendritic cells against human colon cancer cells through upregulation of the transporter associated with antigen processing. Sci Rep 11:9080 (2021). PubMed: 33907276
- Arakawa A et al. ERAP1 Controls the Autoimmune Response against Melanocytes in Psoriasis by Generating the Melanocyte Autoantigen and Regulating Its Amount for HLA-C*06:02 Presentation. J Immunol 207:2235-2244 (2021). PubMed: 34580106
- Wang B et al. p53 increases MHC class I expression by upregulating the endoplasmic reticulum aminopeptidase ERAP1. Nat Commun 4:2359 (2013). WB . PubMed: 23965983