The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution. Allows the detection of at least 0.2 – 0.4 ng/well of recombinant human protein.
sELISA: Use at an assay dependent dilution. Allows the detection of at least 0.2 – 0.4 ng/well of recombinant human protein.
WB: Use at an assay dependent dilution. Detection limit for recombinant human protein is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Predicted molecular weight: 23 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Ligand for the GFR-alpha-3-RET receptor complex but can also activate the GFR-alpha-1-RET receptor complex. Supports the survival of sensory and sympathetic peripheral neurons in culture and also supports the survival of dopaminergic neurons of the ventral mid-brain.
Ubiquitous. Expressed at high levels in peripheral tissues including prostate, placenta, pancreas, heart, kidney, pituitary gland, lung and testis. Expressed at low levels in the brain.
Belongs to the TGF-beta family. GDNF subfamily.
Expressed during embryogenesis. High level expression seen in fetal kidney and lung while a low level expression seen in the fetal brain.