概述

  • 产品名称
    Anti-ART4抗体
  • 描述
    兔多克隆抗体to ART4
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Sheep, Rabbit, Horse, Cow, Pig, Chimpanzee, African green monkey
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 179-228 (TLCYEVHYRT KDVHFNAYTG ATIRFGQFLS TSLLKEEAQE FGNQTLFTIF) of Human ART4 (NP_066549).

  • 阳性对照
    • Human Fetal heart lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab83342 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1/312500.

靶标

  • 相关性
    ART4 is a member of the ADP ribosyltransferase gene family. Antigens of the Dombrock blood group system are located on the gene product, which is glycosylphosphatidylinosotol anchored to the erythrocyte membrane. Allelic variants, some of which lead to adverse transfusion reactions, are known.
  • 细胞定位
    Cell membrane; Lipidbanchor, GPI anchor.
  • 数据库链接
  • 别名
    • ADP ribosyltransferase 4 (DO blood group) antibody
    • ADP ribosyltransferase 4 (Dombrock blood group) antibody
    • ADP ribosyltransferase 4 antibody
    • ART 4 antibody
    • CD297 antibody
    • CD297 antigen antibody
    • DO antibody
    • DOK1 antibody
    • Dombrock blood group carrier molecule antibody
    • Ecto ADP ribosyltransferase 4 antibody
    • Mono ADP ribosyltransferase 4 antibody
    • Mono(ADP ribosyl)transferase 4 antibody
    • NAD(P)(+) arginine ADP ribosyltransferase 4 antibody
    see all

Anti-ART4 antibody 图像

  • Anti-ART4 antibody (ab83342) at 1/1 dilution + fetal heart lysate at 10 µg/ml

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 36 kDa
    Observed band size : 36 kDa

实验方案

Anti-ART4 antibody (ab83342)参考文献

ab83342 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization
Yes - 0.2% Triton X-100
Specification
Heart
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 26 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册