The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 10µl for 106 cells in 100µl.
IP: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
The mouse ADP ribosyltransferase (ART) 2.2 gene is ortholog of rat RT6, and is expressed exclusively by mature T cells. Levels of ART2.2 expression vary between inbred strains of mice and is absent in NZW mice, in which the ART2.2 gene is deleted. ART2.2 has both NAD(+) glycohydrolase and ADP ribosyltransferase activity.