The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 57 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
功能Displays arylsulfatase activity at acidic pH with pseudosubstrates, such as p-nitrocatechol sulfate and also, but with lower activity, p-nitrophenyl sulfate and 4-methylumbelliferyl sulfate.
组织特异性Widely expressed, with very low expression in brain, lung, heart and skeletal muscle.
序列相似性Belongs to the sulfatase family.
翻译后修饰N-glycosylated. The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. Glycosylated.
细胞定位Lysosome. Previously observed endoplasmic reticulum localization is most likely due to folding/maturation problems for overexpressed proteins.