产品名称 Anti-ARRDC4抗体 参阅全部 ARRDC4 一抗
WB, Indirect ELISA, ICC/IF more details
Synthetic peptide derived from an internal region of human ARRDC4.
存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液 Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration information loading...
纯度 Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 45 kDa).
Use at an assay dependent concentration. PubMed: 23236378
序列相似性 Belongs to the arrestin family.
Information by UniProt
ARRDC 4 antibody
Anti-ARRDC4 antibody 图像
Western blot - ARRDC4 antibody (ab74265)
All lanes : Anti-ARRDC4 antibody (ab74265) at 1/500 dilution Lane 1 : extracts from COLO cells Lane 2 : extracts from COLO cells with immunising peptide at 10 µg Lysates/proteins at 30 µg per lane. Predicted band size : 45 kDa Observed band size : 47 kDa ( why is the actual band size different from the predicted?) Additional bands at : 28 kDa,36 kDa. We are unsure as to the identity of these extra bands.
Anti-ARRDC4 antibody (ab74265)参考文献
This product has been referenced in:
et al. Mammalian alpha arrestins link activated seven transmembrane receptors to Nedd4 family e3 ubiquitin ligases and interact with beta arrestins. PLoS One 7:e50557 (2012).
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