Synthetic peptide derived from an internal region of human ARRDC4.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 45 kDa).
Use at an assay dependent concentration. PubMed: 23236378
Belongs to the arrestin family.
Information by UniProt
ARRDC 4 antibody
Western blot - ARRDC4 antibody (ab74265)
All lanes :
Anti-ARRDC4 antibody (ab74265) at 1/500 dilution
Lane 1 :
extracts from COLO cells
Lane 2 :
extracts from COLO cells with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size:
Observed band size:
47 kDa (
why is the actual band size different from the predicted?
Additional bands at:
28 kDa, 36 kDa. We are unsure as to the identity of these extra bands.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"