The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 34 kDa.
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Primary incubation: 1 hour
功能Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament.
ab115200 at 3.75 ug/ml staining ARPC2 in Human Spleen formalin-fixed, paraffin-embedded (FFPE) tissue. Folllowing incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Western blot - Anti-ARPC2 antibody (ab115200)
Anti-ARPC2 antibody (ab115200) at 0.2 µg/ml + HeLa lysate in RIPA buffer at 35 µg developed using the ECL technique
Predicted band size : 34 kDa
Anti-ARPC2 antibody (ab115200)参考文献
has not yet been referenced specifically in any publications.