Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.
Belongs to the WD repeat ARPC1 family. Contains 6 WD repeats.
ab99314 at 1 µg/ml detecting ARP2/3 subunit 1B in HeLa whole cell lysate by WB following IP.
Lane 1: ab99314 at 3µg/mg of lysate
Lane 2: IP with an antibody which recognizes an downstream epitope of ARP2/3 subunit 1B
Lane 3: control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 3 seconds.
Western blot - ARP2/3 subunit 1B antibody (ab99314)
All lanes : Anti-ARP2/3 subunit 1B antibody (ab99314) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T cell lysate at 50 µg Lane 5 : NIH3T3 cell lysate at 50 µg