The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
Use a concentration of 10 µg/ml.
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 40-45 kDa (predicted molecular weight: 45 kDa).
功能Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament.