RabMAb

Anti-Aromatase抗体[EPR4534(2)] (ab124776)

概述

  • 产品名称Anti-Aromatase抗体[EPR4534(2)]
    参阅全部 Aromatase 一抗
  • 描述
    兔单克隆抗体[EPR4534(2)] to Aromatase
  • 经测试应用适用于: WB, IPmore details
    不适用于: Flow Cyt or ICC/IF
  • 种属反应性
    与反应: Rat, Human
    不与反应: Mouse
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Aromatase aa 1-100. The exact sequence is proprietary.
    (Peptide available as ab186919)

  • 阳性对照
    • WB: Human placenta, human uterus, rat brain tissue lysate and JAR and NIH:OVCAR 3 cell lysates. IP: Rat brain tissue lysate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

性能

应用

Our Abpromise guarantee covers the use of ab124776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 58 kDa).Can be blocked with Aromatase peptide (ab186919).
IP 1/10 - 1/300.
  • 应用说明Is unsuitable for Flow Cyt or ICC/IF.
  • 靶标

    • 功能Catalyzes the formation of aromatic C18 estrogens from C19 androgens.
    • 组织特异性Brain, placenta and gonads.
    • 疾病相关Defects in CYP19A1 are a cause of aromatase excess syndrome (AEXS) [MIM:139300]; also known as familial gynecomastia. AEXS is characterized by an estrogen excess due to an increased aromatase activity.
      Defects in CYP19A1 are the cause of aromatase deficiency (AROD) [MIM:107910]. AROD is a rare disease in which fetal androgens are not converted into estrogens due to placental aromatase deficiency. Thus, pregnant women exhibit a hirsutism, which spontaneously resolves after post-partum. At birth, female babies present with pseudohermaphroditism due to virilization of extern genital organs. In adult females, manifestations include delay of puberty, breast hypoplasia and primary amenorrhoea with multicystic ovaries.
    • 序列相似性Belongs to the cytochrome P450 family.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ARO antibody
      • ARO1 antibody
      • Aromatase antibody
      • CP19A_HUMAN antibody
      • CPV1 antibody
      • CYAR antibody
      • CYP19 antibody
      • Cyp19a1 antibody
      • CYPXIX antibody
      • Cytochrome P-450AROM antibody
      • Cytochrome P450 19A1 antibody
      • Cytochrome P450, family 19, subfamily A, polypeptide 1 antibody
      • Cytochrome P450, subfamily XIX (aromatization of androgens) antibody
      • Estrogen synthase antibody
      • Estrogen synthetase antibody
      • Flavoprotein linked monooxygenase antibody
      • MGC104309 antibody
      • Microsomal monooxygenase antibody
      • OTTHUMP00000162543 antibody
      • OTTHUMP00000198350 antibody
      • P 450AROM antibody
      see all

    Anti-Aromatase antibody [EPR4534(2)] 图像

    • Anti-Aromatase antibody [EPR4534(2)] (ab124776) at 1/2000 dilution (purified) + Rat brain tissue lysate at 20 µg

      Secondary
      HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/5000 dilution

      Predicted band size : 58 kDa
      Observed band size : 53 kDa (why is the actual band size different from the predicted?)
      Blocking and dilution buffer: 5% NFDM/TBST
    • Anti-Aromatase antibody [EPR4534(2)] (ab124776) at 1/2000 dilution (purified) + JAR whole cell lysate at 20 µg

      Secondary
      HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/5000 dilution

      Predicted band size : 58 kDa
      Observed band size : 53 kDa (why is the actual band size different from the predicted?)
      Blocking and dilution buffer: 5% NFDM/TBST
    • ab124776 (purified) at a dilution of 1/300 immunoprecipitating Aromatase in rat brain tissue lysate.

      Lane 1 (input): Rat brain tissue lysate (10µg)

      Lane 2 (+): ab124776 + rat brain tissue lysate.

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124776 in rat brain tissue lysate.

      For western blotting, ab131366 VeriBlot for IP (HRP) was used as the secondary antibody (1/1000).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-Aromatase antibody [EPR4534(2)] (ab124776) at 1/1000 dilution (unpurified)

      Lane 1 : Human placenta lysate
      Lane 2 : Human uterus lysate
      Lane 3 : JAR cell lysate
      Lane 4 : NIH:OVCAR 3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 58 kDa
      Observed band size : 53 kDa (why is the actual band size different from the predicted?)
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-Aromatase antibody [EPR4534(2)] (ab124776)参考文献

    This product has been referenced in:
    • Ren J  et al. Estrogen upregulates MICA/B expression in human non-small cell lung cancer through the regulation of ADAM17. Cell Mol Immunol N/A:N/A (2014). Read more (PubMed: 25363527) »

    See 1 Publication for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Hamster Cell lysate - whole cell (BHK17)
    Gel Running Conditions Reduced Denaturing (Gel 10%)
    Loading amount 10 µg
    Specification BHK17
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Simon Boudreault

    Verified customer

    提交于 Mar 17 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"