概述

  • 产品名称
    Anti-Aromatase抗体
    参阅全部 Aromatase 一抗
  • 描述
    兔多克隆抗体to Aromatase
  • 经测试应用
    适用于: WB, ICC/IF, IHC-Fr, IHC-Pmore details
  • 种属反应性
    与反应: Dog, Human
    预测可用于: Rabbit
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human Aromatase.

    (Peptide available as ab35661.)

  • 阳性对照
    • This antibody gave a positive signal in Human placenta tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab35604 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 58 kDa).
ICC/IF Use a concentration of 5 µg/ml.
IHC-Fr 1/50.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能
    Catalyzes the formation of aromatic C18 estrogens from C19 androgens.
  • 组织特异性
    Brain, placenta and gonads.
  • 疾病相关
    Defects in CYP19A1 are a cause of aromatase excess syndrome (AEXS) [MIM:139300]; also known as familial gynecomastia. AEXS is characterized by an estrogen excess due to an increased aromatase activity.
    Defects in CYP19A1 are the cause of aromatase deficiency (AROD) [MIM:107910]. AROD is a rare disease in which fetal androgens are not converted into estrogens due to placental aromatase deficiency. Thus, pregnant women exhibit a hirsutism, which spontaneously resolves after post-partum. At birth, female babies present with pseudohermaphroditism due to virilization of extern genital organs. In adult females, manifestations include delay of puberty, breast hypoplasia and primary amenorrhoea with multicystic ovaries.
  • 序列相似性
    Belongs to the cytochrome P450 family.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ARO antibody
    • ARO1 antibody
    • Aromatase antibody
    • CP19A_HUMAN antibody
    • CPV1 antibody
    • CYAR antibody
    • CYP19 antibody
    • Cyp19a1 antibody
    • CYPXIX antibody
    • Cytochrome P-450AROM antibody
    • Cytochrome P450 19A1 antibody
    • Cytochrome P450, family 19, subfamily A, polypeptide 1 antibody
    • Cytochrome P450, subfamily XIX (aromatization of androgens) antibody
    • Estrogen synthase antibody
    • Estrogen synthetase antibody
    • Flavoprotein linked monooxygenase antibody
    • MGC104309 antibody
    • Microsomal monooxygenase antibody
    • OTTHUMP00000162543 antibody
    • OTTHUMP00000198350 antibody
    • P 450AROM antibody
    see all

Anti-Aromatase antibody 图像

  • Anti-Aromatase antibody (ab35604) at 1 µg/ml + Human placenta tissue lysate - total protein (ab29745) at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 58 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 150 seconds

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab35604 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

  • ab35604 staining Aromatase in dog ovary tissue section by Immunohistochemistry (Frozen sections). Tissue underwent fixation in acetone for 10 minutes and blocked in TBS buffer (1%BSA + 10%NGS) for 2 hours at 25°C. The primary antibody was diluted 1/50 and incubated with sample for 16 hours at 4°C. A HRP conjugated goat polyclonal to rabbit IgG (H&L), diluted 1/100 was used as secondary.

    See Abreview

  • ICC/IF image of ab35604 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab35604, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) HeLa cells at 5µg/ml.

Anti-Aromatase antibody (ab35604)参考文献

This product has been referenced in:
  • Guven S  et al. Functional maintenance of differentiated embryoid bodies in microfluidic systems: a platform for personalized medicine. Stem Cells Transl Med 4:261-8 (2015). ICC/IF ; Mouse . Read more (PubMed: 25666845) »
  • Lim HY  et al. Effects of Obesity and Obesity-Related Molecules on Canine Mammary Gland Tumors. Vet Pathol 52:1045-51 (2015). IHC-P ; Dog . Read more (PubMed: 25883120) »

See all 9 Publications for this product

Product Wall

Application
Western blot
Sample
Human Tissue lysate - whole (Pooled endometrial tissue)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
Pooled endometrial tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Jun 30 2017

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Sample
Mouse Cell (Mouse Ovarian Tumor)
Specification
Mouse Ovarian Tumor
Permeabilization
Yes - 0.1% Triton X
Fixative
NBF
Username

Dr. Athilakshmi Kannan

Verified customer

提交于 Mar 10 2014

Thanks for your enquiry.

It seems that we have already received an enquiry regarding this same issue in July. A refund was given.

I hope this is helpful. Please contact us again if you need anything further.

Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact ...

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Thank you for your reply.

I am sorry to hear that the protocol suggestions did not improve the customer's results. I have found your order and would like to offer you a free of charge replacement antibody or a credit note/refund. Please let m...

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Thank you very much for your interest in our antibodies. For c-Fos, I would recommend either ab7963 or ab87655.  Both of these antibodies will be guaranteed for use in IHC on mouse samples.  For Aromatase, ab18995 and ab35604 will be guaranteed for you...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Dog Tissue sections (ovary)
Specification
ovary
Fixative
Acetone
Permeabilization
Yes - acetone 10 min
Blocking step
1%BSA, 10%NGS in TBS as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Dr. David Matlack

Verified customer

提交于 Dec 17 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (spleen)
Specification
spleen
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6.0
Permeabilization
No
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Aug 22 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (spleen)
Specification
spleen
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6.0
Permeabilization
No
Blocking step
Sequential peroxidase (5min) and protein (10min) block (prediluted). Reagents from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Aug 19 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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