概述

  • 产品名称
    Anti-Aromatase抗体
    参阅全部 Aromatase 一抗
  • 描述
    兔多克隆抗体to Aromatase
  • 特异性
    ab18995 recognises aromatase.
  • 经测试应用
    适用于: ICC/IF, IHC-Fr, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chicken, Cow, Pig
  • 免疫原

    Synthetic peptide surrounding amino acid 385 of human Aromatase (Peptide available as ab51924.)

  • 阳性对照
    • ICC/IF: HepG2 cells. IHC-P: Human normal placenta.

性能

应用

Our Abpromise guarantee covers the use of ab18995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
IHC-Fr Use at an assay dependent concentration. PubMed: 19620711
IHC-P Use a concentration of 10 - 20 µg/ml.
WB Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 58 kDa).Can be blocked with Aromatase peptide (ab51924). An additional 35 kDa band can also be detected in Jurkat cells (we are unsure of the identity).

靶标

  • 功能
    Catalyzes the formation of aromatic C18 estrogens from C19 androgens.
  • 组织特异性
    Brain, placenta and gonads.
  • 疾病相关
    Defects in CYP19A1 are a cause of aromatase excess syndrome (AEXS) [MIM:139300]; also known as familial gynecomastia. AEXS is characterized by an estrogen excess due to an increased aromatase activity.
    Defects in CYP19A1 are the cause of aromatase deficiency (AROD) [MIM:107910]. AROD is a rare disease in which fetal androgens are not converted into estrogens due to placental aromatase deficiency. Thus, pregnant women exhibit a hirsutism, which spontaneously resolves after post-partum. At birth, female babies present with pseudohermaphroditism due to virilization of extern genital organs. In adult females, manifestations include delay of puberty, breast hypoplasia and primary amenorrhoea with multicystic ovaries.
  • 序列相似性
    Belongs to the cytochrome P450 family.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ARO antibody
    • ARO1 antibody
    • Aromatase antibody
    • CP19A_HUMAN antibody
    • CPV1 antibody
    • CYAR antibody
    • CYP19 antibody
    • Cyp19a1 antibody
    • CYPXIX antibody
    • Cytochrome P-450AROM antibody
    • Cytochrome P450 19A1 antibody
    • Cytochrome P450, family 19, subfamily A, polypeptide 1 antibody
    • Cytochrome P450, subfamily XIX (aromatization of androgens) antibody
    • Estrogen synthase antibody
    • Estrogen synthetase antibody
    • Flavoprotein linked monooxygenase antibody
    • MGC104309 antibody
    • Microsomal monooxygenase antibody
    • OTTHUMP00000162543 antibody
    • OTTHUMP00000198350 antibody
    • P 450AROM antibody
    see all

图片

  • Ab18995 staining Human normal placenta. Staining is localised to cell membrane.
    Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • ICC/IF image of ab18995 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18995, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-Aromatase antibody (ab18995) at 1 µg/ml + Human brain normal tissue lysate - membrane extract (ab29456) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
    Additional bands at : 34 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

文献

This product has been referenced in:
  • Dos Santos AC  et al. Immunolocalization of steroidogenic enzymes in the vaginal mucous of Galea spixii during the estrous cycle. Reprod Biol Endocrinol 15:30 (2017). Read more (PubMed: 28438170) »
  • Zhang F  et al. Seasonal changes of androgen receptor, estrogen receptors and aromatase expression in the medial preoptic area of the wild male ground squirrels (Citellus dauricus Brandt). Eur J Histochem 60:2621 (2016). IHC . Read more (PubMed: 27349316) »

See all 21 Publications for this product

客户评价及客户问答

Filter by Application

Filter by Species

Filter by Ratings

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Rat Placenta)
Antigen retrieval step
Enzymatic
Permeabilization
No
Specification
Rat Placenta
Blocking step
Serum as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 1.5%
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 29 2016

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Spinal cord)
Antigen retrieval step
None
Permeabilization
Yes - TritonX 100
Specification
Spinal cord
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 29 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (pancreatic tumor)
Specification
pancreatic tumor
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Oct 08 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (pancreatic tumor)
Specification
pancreatic tumor
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Oct 05 2012

Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - other (brain)
Loading amount
15 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 28 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Spinal Cord membrane)
Loading amount
15 µg
Specification
Spinal Cord membrane
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Vijaya Murugaiyan

Verified customer

提交于 Mar 24 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (pancreatic cells from Estrogen Receptor+ tumor)
Specification
pancreatic cells from Estrogen Receptor+ tumor
Fixative
Formaldehyde
Permeabilization
Yes - PBS + Triton 0.025%
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Oct 01 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册