概述

  • 产品名称Anti-Aromatase抗体
    参阅全部 Aromatase 一抗
  • 描述
    兔多克隆抗体to Aromatase
  • 特异性ab18995 recognises aromatase.
  • 经测试应用适用于: ICC/IF, IHC-Fr, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chicken, Cow, Pig
  • 免疫原

    Synthetic peptide surrounding amino acid 385 of human Aromatase (Peptide available as ab51924.)

  • 阳性对照
    • Brain, placenta and gonads.

性能

应用

Our Abpromise guarantee covers the use of ab18995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
IHC-Fr Use at an assay dependent concentration. PubMed: 19620711
IHC-P Use a concentration of 10 - 20 µg/ml.
WB Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 58 kDa).Can be blocked with Aromatase peptide (ab51924). An additional 35 kDa band can also be detected in Jurkat cells (we are unsure of the identity).

靶标

  • 功能Catalyzes the formation of aromatic C18 estrogens from C19 androgens.
  • 组织特异性Brain, placenta and gonads.
  • 疾病相关Defects in CYP19A1 are a cause of aromatase excess syndrome (AEXS) [MIM:139300]; also known as familial gynecomastia. AEXS is characterized by an estrogen excess due to an increased aromatase activity.
    Defects in CYP19A1 are the cause of aromatase deficiency (AROD) [MIM:107910]. AROD is a rare disease in which fetal androgens are not converted into estrogens due to placental aromatase deficiency. Thus, pregnant women exhibit a hirsutism, which spontaneously resolves after post-partum. At birth, female babies present with pseudohermaphroditism due to virilization of extern genital organs. In adult females, manifestations include delay of puberty, breast hypoplasia and primary amenorrhoea with multicystic ovaries.
  • 序列相似性Belongs to the cytochrome P450 family.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ARO antibody
    • ARO1 antibody
    • Aromatase antibody
    • CP19A_HUMAN antibody
    • CPV1 antibody
    • CYAR antibody
    • CYP19 antibody
    • Cyp19a1 antibody
    • CYPXIX antibody
    • Cytochrome P-450AROM antibody
    • Cytochrome P450 19A1 antibody
    • Cytochrome P450, family 19, subfamily A, polypeptide 1 antibody
    • Cytochrome P450, subfamily XIX (aromatization of androgens) antibody
    • Estrogen synthase antibody
    • Estrogen synthetase antibody
    • Flavoprotein linked monooxygenase antibody
    • MGC104309 antibody
    • Microsomal monooxygenase antibody
    • OTTHUMP00000162543 antibody
    • OTTHUMP00000198350 antibody
    • P 450AROM antibody
    see all

Anti-Aromatase antibody 图像

  • ICC/IF image of ab18995 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18995, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Ab18995 staining Human normal placenta. Staining is localised to cell membrane.
    Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • Anti-Aromatase antibody (ab18995) at 1 µg/ml + Human brain normal tissue lysate - membrane extract (ab29456) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
    Additional bands at : 34 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

Anti-Aromatase antibody (ab18995)参考文献

This product has been referenced in:
  • Khan KN  et al. Occult microscopic endometriosis: undetectable by laparoscopy in normal peritoneum. Hum Reprod 29:462-72 (2014). Human . Read more (PubMed: 24352888) »
  • Dutta S  et al. The steroid hormone environment during primordial follicle formation in perinatal mouse ovaries. Biol Reprod 91:68 (2014). Mouse . Read more (PubMed: 25078683) »

See all 12 Publications for this product

Product Wall

Thank you for getting back to me.

I have seen the correspondence you have had with my colleague Claudia over the ab4360 slide, she wrote the following:

These slides do not automatically contain this region (syncytiotrophoblast area)...

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Thank you for contacting us and your interest in our products. In answer to your questions:

1. Yes, rabbit polyclonal IgG isotype ab27478 would be an appropriate isotype control for the experiment you are hoping to perform with anti- Cytochro...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Rat Placenta)
Antigen retrieval step Enzymatic
Permeabilization No
Specification Rat Placenta
Blocking step Serum as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 1.5%
Fixative Formaldehyde
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Abcam user community

Verified customer

提交于 Mar 29 2016

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Spinal cord)
Antigen retrieval step None
Permeabilization Yes - TritonX 100
Specification Spinal cord
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 29 2015



I have had a look at the western blot from the other distributor and it does indeed show two bands at similar molecular weights to the ones shown on our datasheet. We have also looked at similar antibodies from other companies and their antib...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (pancreatic tumor)
Specification pancreatic tumor
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Oct 08 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (pancreatic tumor)
Specification pancreatic tumor
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Oct 05 2012

Thank you very much for your interest in ab18995.

To our knowledge, ab18995 has not been tested in fish. We do not carry any sample sizes due to our extremely large catalog of products. We do however have a testing discount program. Therefor...

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Thank you your reply.

Your credit note ID is **.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purcha...

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Thank you for your patience, whilst I was talking to the lab about your results.

We have western blot images for this antibody, only from brain lysates. We do not have the same negative controls like you used (ie a KO mouse). We are looking in...

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