概述

  • 产品名称Anti-ARL1抗体
    参阅全部 ARL1 一抗
  • 描述
    兔多克隆抗体to ARL1
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Cow, Pig, Xenopus laevis, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human ARL1.

    (Peptide available as ab88216.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HepG2; HeLa.

性能

应用

Our Abpromise guarantee covers the use of ab76156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
ICC/IF 1/500. Use with paraformaldehyde or methanol fixed cells.

靶标

  • 功能GTP-binding protein that has very low efficiency as allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Can activate phospholipase D with very low efficiency. Important for normal function of the Golgi apparatus.
  • 组织特异性Detected in heart, liver, lung and liver (at protein level). Detected in fetal heart, lung, liver and kidney. Detected in adult heart, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • 序列相似性Belongs to the small GTPase superfamily. Arf family.
  • 细胞定位Golgi apparatus membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADP ribosylation factor like 1 antibody
    • ADP-ribosylation factor-like protein 1 antibody
    • ARFL1 antibody
    • Arl1 antibody
    • ARL1_HUMAN antibody
    see all

Anti-ARL1 antibody 图像

  • All lanes : Anti-ARL1 antibody (ab76156) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 20 kDa
    Observed band size : 20 kDa
    Additional bands at : 58 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes
  • ab76156 (1/500) staining ARL1 in assynchronous HeLa cells (green). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please see Abreview.

    See Abreview

Anti-ARL1 antibody (ab76156)参考文献

ab76156 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100
Username

Dr. Kirk McManus

Verified customer

提交于 Aug 03 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"