概述

  • 产品名称
  • 描述
    兔多克隆抗体to ARK5
  • 经测试应用
    适用于: ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide from the C-terminal region of human ARK5 conjugated to KLH

  • 阳性对照
    • Mouse brain tissue lysate and human breast carcinoma tissue.

性能

应用

Our Abpromise guarantee covers the use of ab71814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).

靶标

  • 功能
    Involved in tolerance to glucose starvation. Phosphorylates ATM. Suppresses Fas-induced apoptosis by phosphorylation of CASP6, thus suppressing the activation of the caspase and the subsequent cleavage of CFLAR.
  • 组织特异性
    Expressed at high levels in heart and brain, and at lower levels in skeletal muscle, kidney, ovary, placenta, lung and liver.
  • 序列相似性
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    Ubiquitinated with 'Lys-29'- and 'Lys-33'-linked polyubiquitins which appear to impede LKB1-mediated phosphorylation. Deubiquitinated by USP9X.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AMP activated protein kinase family member 5 antibody
    • AMPK related protein kinase 5 antibody
    • AMPK-related protein kinase 5 antibody
    • NUAK family SNF1 like kinase antibody
    • NUAK family SNF1-like kinase 1 antibody
    • Nuak1 antibody
    • NUAK1_HUMAN antibody
    • Omphalocele kinase 1 antibody
    • Probable serine/threonine protein kinase KIAA0537 antibody
    see all

图片

  • Anti-ARK5 antibody (ab71814) at 1/100 dilution + mouse brain tissue lysate at 12.5 µg

    Secondary
    HRP-anti-rabbit
    Developed using the ECL technique

    Predicted band size : 74 kDa
    Observed band size : 74 kDa
    Additional bands at : 17 kDa,40 kDa. We are unsure as to the identity of these extra bands.
  • ab71814 at 1/50 dilution, staining ARK5 in human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

文献

This product has been referenced in:
  • Lasagna-Reeves CA  et al. Reduction of Nuak1 Decreases Tau and Reverses Phenotypes in a Tauopathy Mouse Model. Neuron 92:407-418 (2016). WB . Read more (PubMed: 27720485) »

See 1 Publication for this product

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