概述

  • 产品名称
  • 描述
    兔多克隆抗体to ARK5
  • 经测试应用
    适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Hamster, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to sequence from within N terminal amino acids 1-100 of Human ARK5.

  • 阳性对照
    • Mouse brain tissue lysate, Human breast carcinoma.

性能

应用

Our Abpromise guarantee covers the use of ab37641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
IHC-P Use at an assay dependent concentration.

靶标

  • 功能
    Involved in tolerance to glucose starvation. Phosphorylates ATM. Suppresses Fas-induced apoptosis by phosphorylation of CASP6, thus suppressing the activation of the caspase and the subsequent cleavage of CFLAR.
  • 组织特异性
    Expressed at high levels in heart and brain, and at lower levels in skeletal muscle, kidney, ovary, placenta, lung and liver.
  • 序列相似性
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    Ubiquitinated with 'Lys-29'- and 'Lys-33'-linked polyubiquitins which appear to impede LKB1-mediated phosphorylation. Deubiquitinated by USP9X.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AMP activated protein kinase family member 5 antibody
    • AMPK related protein kinase 5 antibody
    • AMPK-related protein kinase 5 antibody
    • NUAK family SNF1 like kinase antibody
    • NUAK family SNF1-like kinase 1 antibody
    • Nuak1 antibody
    • NUAK1_HUMAN antibody
    • Omphalocele kinase 1 antibody
    • Probable serine/threonine protein kinase KIAA0537 antibody
    see all

图片

  • Anti-ARK5 antibody (ab37641) at 1/100 dilution + Mouse brain tissue lysate

    Predicted band size : 74 kDa
    Observed band size : 74 kDa
  • ab37641, at 1/50 dilution, staining ARK5 in Human breast carcinoma by Immunohistochemistry, Formalin fixed, Paraffin embedded tissue. ab37641 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

文献

ab37641 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Blocking step
Odyssey block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
Username

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提交于 Apr 24 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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